Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats

BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile durin...

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Autores principales:	Chen,Xiao-Guang, Xu,Cun-Shuan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Partial hepatectomy Rat liver regeneration Proteomic MALDI TOF/TOF MS
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100053
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spelling	oai:scielo:S0716-976020140001000532015-10-30Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in ratsChen,Xiao-GuangXu,Cun-Shuan Partial hepatectomy Rat liver regeneration Proteomic MALDI TOF/TOF MS BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR has been rarely reported up to date. Therefore, this study aims to detect the global proteomic profile of the regenerating rat liver following 2/3 hepatectomy, thereby gaining some insights into hepatic regeneration mechanism. RESULTS: Protein samples extracted from the sham-operated and the regenerating rat livers at 6, 12, 24, 72, 120 and 168 h after PH were separated by IEF/SDS-PAGE and then analyzed by MALDI-TOF/TOF mass spectrometry. Compared to sham-operated groups, there were totally 220 differentially expressed proteins (including 156 up-regulated, 62 down-regulated, and 2 up/down-regulated ones) identified in the regenerating rat livers, and most of them have not been previously related to liver regeneration. According to the expression pattern analysis combined with gene functional analysis, it showed that lipid and carbohydrate metabolism were enhanced at the early phase of LR and continue throughout the regeneration process. Ingenuity Pathway Analysis indicated that YWHAE protein (one of members of the 14-3-3 protein family) was located at the center of pathway networks at all the timepoints after 2/3 hepatectomy under our experimental conditions, maybe suggesting a central role of this protein in regulating liver regeneration. Additionally, we also revealed the role of Cdc42 (cell division cycle 42) in the termination of LR. CONCLUSIONS: For the first time, our proteomic analysis suggested an important role of YWHAE and pathway mediated by this protein in liver regeneration, which might be helpful in expanding our understanding of LR amd unraveling the mechanisms of LR.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100053en10.1186/0717-6287-47-59
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Partial hepatectomy Rat liver regeneration Proteomic MALDI TOF/TOF MS
spellingShingle	Partial hepatectomy Rat liver regeneration Proteomic MALDI TOF/TOF MS Chen,Xiao-Guang Xu,Cun-Shuan Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
description	BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR has been rarely reported up to date. Therefore, this study aims to detect the global proteomic profile of the regenerating rat liver following 2/3 hepatectomy, thereby gaining some insights into hepatic regeneration mechanism. RESULTS: Protein samples extracted from the sham-operated and the regenerating rat livers at 6, 12, 24, 72, 120 and 168 h after PH were separated by IEF/SDS-PAGE and then analyzed by MALDI-TOF/TOF mass spectrometry. Compared to sham-operated groups, there were totally 220 differentially expressed proteins (including 156 up-regulated, 62 down-regulated, and 2 up/down-regulated ones) identified in the regenerating rat livers, and most of them have not been previously related to liver regeneration. According to the expression pattern analysis combined with gene functional analysis, it showed that lipid and carbohydrate metabolism were enhanced at the early phase of LR and continue throughout the regeneration process. Ingenuity Pathway Analysis indicated that YWHAE protein (one of members of the 14-3-3 protein family) was located at the center of pathway networks at all the timepoints after 2/3 hepatectomy under our experimental conditions, maybe suggesting a central role of this protein in regulating liver regeneration. Additionally, we also revealed the role of Cdc42 (cell division cycle 42) in the termination of LR. CONCLUSIONS: For the first time, our proteomic analysis suggested an important role of YWHAE and pathway mediated by this protein in liver regeneration, which might be helpful in expanding our understanding of LR amd unraveling the mechanisms of LR.
author	Chen,Xiao-Guang Xu,Cun-Shuan
author_facet	Chen,Xiao-Guang Xu,Cun-Shuan
author_sort	Chen,Xiao-Guang
title	Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
title_short	Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
title_full	Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
title_fullStr	Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
title_full_unstemmed	Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
title_sort	proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100053
work_keys_str_mv	AT chenxiaoguang proteomicanalysisoftheregeneratingliverfollowing23partialhepatectomyinrats AT xucunshuan proteomicanalysisoftheregeneratingliverfollowing23partialhepatectomyinrats
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Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats

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