Expression of non-neuronal cholinergic system in maxilla of rat in vivo

BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULT...

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Autores principales:	Guo,Jie, Wang,Lue, Xu,Haihua, Che,Xiaoxia
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Non-neuronal cholinergic system Maxilla Real-Time PCR Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066
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spelling	oai:scielo:S0716-976020140001000662015-10-30Expression of non-neuronal cholinergic system in maxilla of rat in vivoGuo,JieWang,LueXu,HaihuaChe,Xiaoxia Non-neuronal cholinergic system Maxilla Real-Time PCR Immunohistochemistry BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, β1, β2, β4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits α2, β1, β4 and mAChR subunits M4 had abundant expression (2-ΔCt > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. CONCLUSIONS: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066en10.1186/0717-6287-47-72
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Non-neuronal cholinergic system Maxilla Real-Time PCR Immunohistochemistry
spellingShingle	Non-neuronal cholinergic system Maxilla Real-Time PCR Immunohistochemistry Guo,Jie Wang,Lue Xu,Haihua Che,Xiaoxia Expression of non-neuronal cholinergic system in maxilla of rat in vivo
description	BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, β1, β2, β4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits α2, β1, β4 and mAChR subunits M4 had abundant expression (2-ΔCt > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. CONCLUSIONS: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.
author	Guo,Jie Wang,Lue Xu,Haihua Che,Xiaoxia
author_facet	Guo,Jie Wang,Lue Xu,Haihua Che,Xiaoxia
author_sort	Guo,Jie
title	Expression of non-neuronal cholinergic system in maxilla of rat in vivo
title_short	Expression of non-neuronal cholinergic system in maxilla of rat in vivo
title_full	Expression of non-neuronal cholinergic system in maxilla of rat in vivo
title_fullStr	Expression of non-neuronal cholinergic system in maxilla of rat in vivo
title_full_unstemmed	Expression of non-neuronal cholinergic system in maxilla of rat in vivo
title_sort	expression of non-neuronal cholinergic system in maxilla of rat in vivo
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066
work_keys_str_mv	AT guojie expressionofnonneuronalcholinergicsysteminmaxillaofratinvivo AT wanglue expressionofnonneuronalcholinergicsysteminmaxillaofratinvivo AT xuhaihua expressionofnonneuronalcholinergicsysteminmaxillaofratinvivo AT chexiaoxia expressionofnonneuronalcholinergicsysteminmaxillaofratinvivo
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Expression of non-neuronal cholinergic system in maxilla of rat in vivo

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