Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells

BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In...

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Autores principales:	Yin,Lei-Miao, Wei,Ying, Wang,Wen-Qian, Wang,Yu, Xu,Yu-Dong, Yang,Yong-Qing
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	BrdU WST-1 Cell proliferation Cell viability Airway smooth muscle cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069
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spelling	oai:scielo:S0716-976020140001000692015-10-30Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cellsYin,Lei-MiaoWei,YingWang,Wen-QianWang,YuXu,Yu-DongYang,Yong-Qing BrdU WST-1 Cell proliferation Cell viability Airway smooth muscle cells BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells. CONCLUSIONS: Our study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069en10.1186/0717-6287-47-75
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	BrdU WST-1 Cell proliferation Cell viability Airway smooth muscle cells
spellingShingle	BrdU WST-1 Cell proliferation Cell viability Airway smooth muscle cells Yin,Lei-Miao Wei,Ying Wang,Wen-Qian Wang,Yu Xu,Yu-Dong Yang,Yong-Qing Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
description	BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells. CONCLUSIONS: Our study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.
author	Yin,Lei-Miao Wei,Ying Wang,Wen-Qian Wang,Yu Xu,Yu-Dong Yang,Yong-Qing
author_facet	Yin,Lei-Miao Wei,Ying Wang,Wen-Qian Wang,Yu Xu,Yu-Dong Yang,Yong-Qing
author_sort	Yin,Lei-Miao
title	Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
title_short	Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
title_full	Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
title_fullStr	Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
title_full_unstemmed	Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
title_sort	simultaneous application of brdu and wst-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069
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Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells

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