Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells

BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bae,Seunghee, Lim,Kyung Mi, Cha,Hwa Jun, An,In-Sook, Lee,Jeong Pyo, Lee,Kwang Sik, Lee,Ghang Tai, Lee,Kun Kook, Jung,Ho Jung, Ahn,Kyu Joong, An,Sungkwan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Dermal papilla cell Senescence Cell death microRNA Arctiin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100073
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602014000100073
record_format	dspace
spelling	oai:scielo:S0716-976020140001000732015-10-30Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cellsBae,SeungheeLim,Kyung MiCha,Hwa JunAn,In-SookLee,Jeong PyoLee,Kwang SikLee,Ghang TaiLee,Kun KookJung,Ho JungAhn,Kyu JoongAn,Sungkwan Dermal papilla cell Senescence Cell death microRNA Arctiin BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell viability. Moreover, H2O2-induced sub-G1 phase accumulation and G2 cell cycle arrest were also downregulated by arctiin pretreatment. Interestingly, the increase in intracellular ROS mediated by H2O2 was drastically decreased in HHDPCs cultured in the presence of arctiin. This effect was confirmed by senescence associated-beta galactosidase (SA-β-gal) assay results; we found that arctiin pretreatment impaired H2O2-induced senescence in HHDPCs. Using microRNA (miRNA) microarray and bioinformatic analysis, we showed that this anti-oxidative effect of arctiin in HHDPCs was related with mitogen-activated protein kinase (MAPK) and Wnt signaling pathways. CONCLUSIONS: Taken together, our data suggest that arctiin has a protective effect on ROS-induced cell dysfunction in HHDPCs and may therefore be useful for alopecia prevention and treatment strategies.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100073en10.1186/0717-6287-47-50
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dermal papilla cell Senescence Cell death microRNA Arctiin
spellingShingle	Dermal papilla cell Senescence Cell death microRNA Arctiin Bae,Seunghee Lim,Kyung Mi Cha,Hwa Jun An,In-Sook Lee,Jeong Pyo Lee,Kwang Sik Lee,Ghang Tai Lee,Kun Kook Jung,Ho Jung Ahn,Kyu Joong An,Sungkwan Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
description	BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell viability. Moreover, H2O2-induced sub-G1 phase accumulation and G2 cell cycle arrest were also downregulated by arctiin pretreatment. Interestingly, the increase in intracellular ROS mediated by H2O2 was drastically decreased in HHDPCs cultured in the presence of arctiin. This effect was confirmed by senescence associated-beta galactosidase (SA-β-gal) assay results; we found that arctiin pretreatment impaired H2O2-induced senescence in HHDPCs. Using microRNA (miRNA) microarray and bioinformatic analysis, we showed that this anti-oxidative effect of arctiin in HHDPCs was related with mitogen-activated protein kinase (MAPK) and Wnt signaling pathways. CONCLUSIONS: Taken together, our data suggest that arctiin has a protective effect on ROS-induced cell dysfunction in HHDPCs and may therefore be useful for alopecia prevention and treatment strategies.
author	Bae,Seunghee Lim,Kyung Mi Cha,Hwa Jun An,In-Sook Lee,Jeong Pyo Lee,Kwang Sik Lee,Ghang Tai Lee,Kun Kook Jung,Ho Jung Ahn,Kyu Joong An,Sungkwan
author_facet	Bae,Seunghee Lim,Kyung Mi Cha,Hwa Jun An,In-Sook Lee,Jeong Pyo Lee,Kwang Sik Lee,Ghang Tai Lee,Kun Kook Jung,Ho Jung Ahn,Kyu Joong An,Sungkwan
author_sort	Bae,Seunghee
title	Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
title_short	Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
title_full	Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
title_fullStr	Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
title_full_unstemmed	Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells
title_sort	arctiin blocks hydrogen peroxide-induced senescence and cell death though microrna expression changes in human dermal papilla cells
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100073
work_keys_str_mv	AT baeseunghee arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT limkyungmi arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT chahwajun arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT aninsook arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT leejeongpyo arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT leekwangsik arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT leeghangtai arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT leekunkook arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT junghojung arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT ahnkyujoong arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells AT ansungkwan arctiinblockshydrogenperoxideinducedsenescenceandcelldeaththoughmicrornaexpressionchangesinhumandermalpapillacells
_version_	1718441527439523840

Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells

Ejemplares similares