Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma

BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been r...

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Autores principales:	Shen,Cong-Cong, Kang,Yu-Huan, Yu,Lin, Cui,Dan-Dan, He,Yi, Yang,Jin-Liang, Gou,Lan-Tu
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2014
Materias:	Immunohistochemistry TEX101 Seminoma Testis Western blotting
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075
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spelling	oai:scielo:S0716-976020140001000752015-10-30Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminomaShen,Cong-CongKang,Yu-HuanYu,LinCui,Dan-DanHe,YiYang,Jin-LiangGou,Lan-Tu Immunohistochemistry TEX101 Seminoma Testis Western blotting BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.47 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075en10.1186/0717-6287-47-52
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Immunohistochemistry TEX101 Seminoma Testis Western blotting
spellingShingle	Immunohistochemistry TEX101 Seminoma Testis Western blotting Shen,Cong-Cong Kang,Yu-Huan Yu,Lin Cui,Dan-Dan He,Yi Yang,Jin-Liang Gou,Lan-Tu Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
description	BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.
author	Shen,Cong-Cong Kang,Yu-Huan Yu,Lin Cui,Dan-Dan He,Yi Yang,Jin-Liang Gou,Lan-Tu
author_facet	Shen,Cong-Cong Kang,Yu-Huan Yu,Lin Cui,Dan-Dan He,Yi Yang,Jin-Liang Gou,Lan-Tu
author_sort	Shen,Cong-Cong
title	Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_short	Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_full	Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_fullStr	Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_full_unstemmed	Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_sort	human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
publisher	Sociedad de Biología de Chile
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075
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Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma

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