Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts

BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammator...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bae,Seunghee, Jung,Younjung, Choi,Yeong Min, Li,Shunhua
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Er-Miao-San MMP1 Human dermal fibroblasts TNF-α NF-kB
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602015000100009
record_format	dspace
spelling	oai:scielo:S0716-976020150001000092016-02-16Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblastsBae,SeungheeJung,YounjungChoi,Yeong MinLi,Shunhua Er-Miao-San MMP1 Human dermal fibroblasts TNF-α NF-kB BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. RESULTS: This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as ΙL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-kBpathway. CONCLUSIONS: Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009en10.1186/0717-6287-48-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Er-Miao-San MMP1 Human dermal fibroblasts TNF-α NF-kB
spellingShingle	Er-Miao-San MMP1 Human dermal fibroblasts TNF-α NF-kB Bae,Seunghee Jung,Younjung Choi,Yeong Min Li,Shunhua Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
description	BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. RESULTS: This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as ΙL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-kBpathway. CONCLUSIONS: Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.
author	Bae,Seunghee Jung,Younjung Choi,Yeong Min Li,Shunhua
author_facet	Bae,Seunghee Jung,Younjung Choi,Yeong Min Li,Shunhua
author_sort	Bae,Seunghee
title	Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
title_short	Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
title_full	Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
title_fullStr	Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
title_full_unstemmed	Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
title_sort	effects of er-miao-san extracts on tnf-alpha-indueed mmp-1 expression in human dermal fibroblasts
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009
work_keys_str_mv	AT baeseunghee effectsofermiaosanextractsontnfalphaindueedmmp1expressioninhumandermalfibroblasts AT jungyounjung effectsofermiaosanextractsontnfalphaindueedmmp1expressioninhumandermalfibroblasts AT choiyeongmin effectsofermiaosanextractsontnfalphaindueedmmp1expressioninhumandermalfibroblasts AT lishunhua effectsofermiaosanextractsontnfalphaindueedmmp1expressioninhumandermalfibroblasts
_version_	1718441530053623808

Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts

Ejemplares similares