Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) produc...
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| Lenguaje: | English |
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Sociedad de Biología de Chile
2015
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oai:scielo:S0716-976020150001000122016-02-16Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosusThao,Nguyen PhuongLuyen,Bui Thi ThuyKoo,Jung EunKim,SohyunKoh,Young SangCuong,Nguyen XuanNam,Nguyen HoaiVan Kiem,PhanKim,Young HoVan Minh,Chau Protoreaster nodosus Starfish IL-12 p40 IL-6 TNF-α LPS-stimulated BMDCs Anti-inflammatory BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ±0.01 to 26.19 ±0.64 μg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3β,6α,7α,8β,15α,16β,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 μM), and for (25S)5α-cholestane-3β, 6α,8β, 15α,16β, 26-hexol (1) and (25S)5α-cholestane-3β,6α,7α,8β,15α,16β,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ±0.00 and and 1.02 ± 0.01 μM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100012en10.1186/s40659-015-0002-2 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Protoreaster nodosus Starfish IL-12 p40 IL-6 TNF-α LPS-stimulated BMDCs Anti-inflammatory |
| spellingShingle |
Protoreaster nodosus Starfish IL-12 p40 IL-6 TNF-α LPS-stimulated BMDCs Anti-inflammatory Thao,Nguyen Phuong Luyen,Bui Thi Thuy Koo,Jung Eun Kim,Sohyun Koh,Young Sang Cuong,Nguyen Xuan Nam,Nguyen Hoai Van Kiem,Phan Kim,Young Ho Van Minh,Chau Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| description |
BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ±0.01 to 26.19 ±0.64 μg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3β,6α,7α,8β,15α,16β,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 μM), and for (25S)5α-cholestane-3β, 6α,8β, 15α,16β, 26-hexol (1) and (25S)5α-cholestane-3β,6α,7α,8β,15α,16β,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ±0.00 and and 1.02 ± 0.01 μM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases. |
| author |
Thao,Nguyen Phuong Luyen,Bui Thi Thuy Koo,Jung Eun Kim,Sohyun Koh,Young Sang Cuong,Nguyen Xuan Nam,Nguyen Hoai Van Kiem,Phan Kim,Young Ho Van Minh,Chau |
| author_facet |
Thao,Nguyen Phuong Luyen,Bui Thi Thuy Koo,Jung Eun Kim,Sohyun Koh,Young Sang Cuong,Nguyen Xuan Nam,Nguyen Hoai Van Kiem,Phan Kim,Young Ho Van Minh,Chau |
| author_sort |
Thao,Nguyen Phuong |
| title |
Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| title_short |
Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| title_full |
Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| title_fullStr |
Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| title_full_unstemmed |
Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus |
| title_sort |
anti-inflammatory components of the vietnamese starfish protoreaster nodosus |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2015 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100012 |
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