Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)

BACKGROUND: Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were ra...

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Autores principales:	Cid-Hernández,Margarita, Ramírez-Anguiano,Ana C, Ortiz,Genaro G, Morales-Sanchez,Eddic W, González-Ortiz,Luis J, Velasco-Ramírez,Sandra F, Pacheco-Moisés,Fermín P
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Karwinskia humboldtiana ATPase Mitochondria Membrane fluidity pH gradient
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100017
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spelling	oai:scielo:S0716-976020150001000172016-02-16Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)Cid-Hernández,MargaritaRamírez-Anguiano,Ana COrtiz,Genaro GMorales-Sanchez,Eddic WGonzález-Ortiz,Luis JVelasco-Ramírez,Sandra FPacheco-Moisés,Fermín P Karwinskia humboldtiana ATPase Mitochondria Membrane fluidity pH gradient BACKGROUND: Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were randomly assigned into control non-treated group and groups that received 1,1.5 and 2 g/Kg body weight of dry powder of Kh fruit, respectively. Rats were euthanized at day 1 and 7 after treatment RESULTS: Rats under Kh treatment at all dose levels tested, does not developed any neurologic symptoms. However, we detected alterations in membrane fluidity and ATPase activity. Lower dose of Kh on day 1 after treatment induced higher mitochondrial membrane fluidity than control group. This change was strongly correlated with increased ATPase activity and pH gradient driven by ATP hydrolysis. On the other hand, membrane fluidity was hardly affected on day 7 after treatment with Kh. Surprisingly, the pH gradient driven by ATPase activity was significantly higher than controls despite an diminution of the hydrolytic activity of ATPase CONCLUSIONS: The changes in ATPase activity and pH gradient driven by ATPase activity suggest an adaptive condition whereby the fluidity of the membrane is alteredinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100017en10.1186/s40659-015-0008-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Karwinskia humboldtiana ATPase Mitochondria Membrane fluidity pH gradient
spellingShingle	Karwinskia humboldtiana ATPase Mitochondria Membrane fluidity pH gradient Cid-Hernández,Margarita Ramírez-Anguiano,Ana C Ortiz,Genaro G Morales-Sanchez,Eddic W González-Ortiz,Luis J Velasco-Ramírez,Sandra F Pacheco-Moisés,Fermín P Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
description	BACKGROUND: Karwinskia humboldtiana (Kh) is a poisonous plant of the rhamnacea family. To elucidate some of the subcellular effects of Kh toxicity, membrane fluidity and ATPase activities as hydrolytic and as proton-pumping activity were assessed in rat liver submitochondrial particles. Rats were randomly assigned into control non-treated group and groups that received 1,1.5 and 2 g/Kg body weight of dry powder of Kh fruit, respectively. Rats were euthanized at day 1 and 7 after treatment RESULTS: Rats under Kh treatment at all dose levels tested, does not developed any neurologic symptoms. However, we detected alterations in membrane fluidity and ATPase activity. Lower dose of Kh on day 1 after treatment induced higher mitochondrial membrane fluidity than control group. This change was strongly correlated with increased ATPase activity and pH gradient driven by ATP hydrolysis. On the other hand, membrane fluidity was hardly affected on day 7 after treatment with Kh. Surprisingly, the pH gradient driven by ATPase activity was significantly higher than controls despite an diminution of the hydrolytic activity of ATPase CONCLUSIONS: The changes in ATPase activity and pH gradient driven by ATPase activity suggest an adaptive condition whereby the fluidity of the membrane is altered
author	Cid-Hernández,Margarita Ramírez-Anguiano,Ana C Ortiz,Genaro G Morales-Sanchez,Eddic W González-Ortiz,Luis J Velasco-Ramírez,Sandra F Pacheco-Moisés,Fermín P
author_facet	Cid-Hernández,Margarita Ramírez-Anguiano,Ana C Ortiz,Genaro G Morales-Sanchez,Eddic W González-Ortiz,Luis J Velasco-Ramírez,Sandra F Pacheco-Moisés,Fermín P
author_sort	Cid-Hernández,Margarita
title	Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
title_short	Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
title_full	Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
title_fullStr	Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
title_full_unstemmed	Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)
title_sort	mitochondrial atpase activity and membrane fluidity changes in rat liver in response to intoxication with buckthorn (karwinskia humboldtiana)
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100017
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Mitochondrial ATPase activity and membrane fluidity changes in rat liver in response to intoxication with Buckthorn (Karwinskia humboldtiana)

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