Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro

BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular ca...

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Autores principales:	Pan,Zeya, Pan,Hao, Zhang,Jin, Yang,Yun, Liu,Hui, Yang,Yuan, Huang,Gang, Ni,Junsheng, Huang,Jian, Zhou,Weiping
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Ubiquitin Specific Peptidase 39 Lentivirus Human hepatocellular carcinoma Cell proliferation Cell cycle
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018
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spelling	oai:scielo:S0716-976020150001000182016-02-16Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitroPan,ZeyaPan,HaoZhang,JinYang,YunLiu,HuiYang,YuanHuang,GangNi,JunshengHuang,JianZhou,Weiping Ubiquitin Specific Peptidase 39 Lentivirus Human hepatocellular carcinoma Cell proliferation Cell cycle BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells. CONCLUSIONS: All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018en10.1186/s40659-015-0006-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ubiquitin Specific Peptidase 39 Lentivirus Human hepatocellular carcinoma Cell proliferation Cell cycle
spellingShingle	Ubiquitin Specific Peptidase 39 Lentivirus Human hepatocellular carcinoma Cell proliferation Cell cycle Pan,Zeya Pan,Hao Zhang,Jin Yang,Yun Liu,Hui Yang,Yuan Huang,Gang Ni,Junsheng Huang,Jian Zhou,Weiping Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
description	BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells. CONCLUSIONS: All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC.
author	Pan,Zeya Pan,Hao Zhang,Jin Yang,Yun Liu,Hui Yang,Yuan Huang,Gang Ni,Junsheng Huang,Jian Zhou,Weiping
author_facet	Pan,Zeya Pan,Hao Zhang,Jin Yang,Yun Liu,Hui Yang,Yuan Huang,Gang Ni,Junsheng Huang,Jian Zhou,Weiping
author_sort	Pan,Zeya
title	Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
title_short	Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
title_full	Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
title_fullStr	Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
title_full_unstemmed	Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
title_sort	lentivirus mediated silencing of ubiquitin specific peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018
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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro

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