Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.

Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.

BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitorin...

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Autores principales: NK,Asha Tukappa, Londonkar,Ramesh L, Nayaka,Hanumantappa B, CB,Sanjeev Kumar
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2015
Materias:
Rumex vesicarius L
HepG2
MDA
SOD
CAT
CCl4 induced hepatotocixity
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100019
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spelling oai:scielo:S0716-976020150001000192016-02-16Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.NK,Asha TukappaLondonkar,Ramesh LNayaka,Hanumantappa BCB,Sanjeev Kumar Rumex vesicarius L HepG2 MDA SOD CAT CCl4 induced hepatotocixity BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 Mg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100019en10.1186/s40659-015-0009-8
institution Scielo Chile
collection Scielo Chile
language English
topic Rumex vesicarius L
HepG2
MDA
SOD
CAT
CCl4 induced hepatotocixity
spellingShingle Rumex vesicarius L
HepG2
MDA
SOD
CAT
CCl4 induced hepatotocixity
NK,Asha Tukappa
Londonkar,Ramesh L
Nayaka,Hanumantappa B
CB,Sanjeev Kumar
Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
description BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 Mg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.
author NK,Asha Tukappa
Londonkar,Ramesh L
Nayaka,Hanumantappa B
CB,Sanjeev Kumar
author_facet NK,Asha Tukappa
Londonkar,Ramesh L
Nayaka,Hanumantappa B
CB,Sanjeev Kumar
author_sort NK,Asha Tukappa
title Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
title_short Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
title_full Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
title_fullStr Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
title_full_unstemmed Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.
title_sort cytotoxicity and hepatoprotective attributes of methanolic extract of rumex vesicarius l.
publisher Sociedad de Biología de Chile
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100019
work_keys_str_mv AT nkashatukappa cytotoxicityandhepatoprotectiveattributesofmethanolicextractofrumexvesicariusl
AT londonkarrameshl cytotoxicityandhepatoprotectiveattributesofmethanolicextractofrumexvesicariusl
AT nayakahanumantappab cytotoxicityandhepatoprotectiveattributesofmethanolicextractofrumexvesicariusl
AT cbsanjeevkumar cytotoxicityandhepatoprotectiveattributesofmethanolicextractofrumexvesicariusl
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