Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis

BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence FINDINGS: By using Phenotypic Microarrays (PM) we analyzed nea...

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Autores principales:	Ortiz-Severín,Javiera, Varas,Macarena, Bravo-Toncio,Catalina, Guiliani,Nicolás, Chávez,Francisco P
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Antibiotic susceptibility Polyphosphate kinase Pseudomonas Phenome Phenotypic microarrays Multidrug resistance
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100022
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spelling	oai:scielo:S0716-976020150001000222016-02-16Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysisOrtiz-Severín,JavieraVaras,MacarenaBravo-Toncio,CatalinaGuiliani,NicolásChávez,Francisco P Antibiotic susceptibility Polyphosphate kinase Pseudomonas Phenome Phenotypic microarrays Multidrug resistance BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence FINDINGS: By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin CONCLUSIONS: Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100022en10.1186/s40659-015-0012-0
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antibiotic susceptibility Polyphosphate kinase Pseudomonas Phenome Phenotypic microarrays Multidrug resistance
spellingShingle	Antibiotic susceptibility Polyphosphate kinase Pseudomonas Phenome Phenotypic microarrays Multidrug resistance Ortiz-Severín,Javiera Varas,Macarena Bravo-Toncio,Catalina Guiliani,Nicolás Chávez,Francisco P Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
description	BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence FINDINGS: By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin CONCLUSIONS: Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.
author	Ortiz-Severín,Javiera Varas,Macarena Bravo-Toncio,Catalina Guiliani,Nicolás Chávez,Francisco P
author_facet	Ortiz-Severín,Javiera Varas,Macarena Bravo-Toncio,Catalina Guiliani,Nicolás Chávez,Francisco P
author_sort	Ortiz-Severín,Javiera
title	Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
title_short	Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
title_full	Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
title_fullStr	Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
title_full_unstemmed	Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
title_sort	multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from pseudomonas aeruginosa pao1 as revealed by global phenotypic analysis
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100022
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Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis

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