Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture

BACKGROUND: The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromy...

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Autores principales:	Chen,Peng, Wang,Yuxia, Yan,Lei, Wang,Yiqing, Li,Suyue, Yan,Xiaojuan, Wang,Ningbo, Liang,Ning, Li,Hongyu
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Renewable Energy Biohydrogen Microbial consortium Hydrogen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100024
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spelling	oai:scielo:S0716-976020150001000242016-02-16Feasibility of biohydrogen production from industrial wastes using defined microbial co-cultureChen,PengWang,YuxiaYan,LeiWang,YiqingLi,SuyueYan,XiaojuanWang,NingboLiang,NingLi,Hongyu Renewable Energy Biohydrogen Microbial consortium Hydrogen BACKGROUND: The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step biohydrogen production experiment, H1 medium (nutrient broth and potato dextrose broth) was mixed directly with GAM broth to generate H2 medium (H1 medium and GAM broth). Finally, Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D of three species microbial co-culture to produce hydrogen under anaerobic conditions. For the two-step biohydrogen production experiment, the H1 medium, after cultured the microbial strains Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, was centrifuged to remove the microbial cells and then mixed with GAM broth (H2 medium). Afterward, the bacterial strain Clostridium acetobutylicum ATCC 824 was inoculated into the H2 medium to produce hydrogen by anaerobic fermentation. RESULTS: The experimental results demonstrated that the optimum conditions for the small-scale fermentative hydrogen production system were at pH 7.0, 35°C, a mixed medium, including H1 medium and H2 medium with 0.50 mol/L ferrous chloride, 0.50 mol/L magnesium sulfate, 0.50 mol/L potassium chloride, 1% w/v citric acid, 5% w/v fructose and 5% w/v glucose. The overall hydrogen production efficiency in the shake flask fermentation group was 33.7 mL/h-1.L-1, and those the two-step and the one-step processes of the small-scale fermentative hydrogen production system were 41.2 mLVh-1.L-1 and 35.1 mL/h-1.L-1, respectively. CONCLUSION: Therefore, the results indicate that the hydrogen production efficiency of the two-step process is higher than that of the one-step process.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100024en10.1186/s40659-015-0015-x
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Renewable Energy Biohydrogen Microbial consortium Hydrogen
spellingShingle	Renewable Energy Biohydrogen Microbial consortium Hydrogen Chen,Peng Wang,Yuxia Yan,Lei Wang,Yiqing Li,Suyue Yan,Xiaojuan Wang,Ningbo Liang,Ning Li,Hongyu Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
description	BACKGROUND: The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step biohydrogen production experiment, H1 medium (nutrient broth and potato dextrose broth) was mixed directly with GAM broth to generate H2 medium (H1 medium and GAM broth). Finally, Clostridium acetobutylicum ATCC 824, Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D of three species microbial co-culture to produce hydrogen under anaerobic conditions. For the two-step biohydrogen production experiment, the H1 medium, after cultured the microbial strains Enterobacter cloacae ATCC 13047 and Kluyveromyces marxianus 15D, was centrifuged to remove the microbial cells and then mixed with GAM broth (H2 medium). Afterward, the bacterial strain Clostridium acetobutylicum ATCC 824 was inoculated into the H2 medium to produce hydrogen by anaerobic fermentation. RESULTS: The experimental results demonstrated that the optimum conditions for the small-scale fermentative hydrogen production system were at pH 7.0, 35°C, a mixed medium, including H1 medium and H2 medium with 0.50 mol/L ferrous chloride, 0.50 mol/L magnesium sulfate, 0.50 mol/L potassium chloride, 1% w/v citric acid, 5% w/v fructose and 5% w/v glucose. The overall hydrogen production efficiency in the shake flask fermentation group was 33.7 mL/h-1.L-1, and those the two-step and the one-step processes of the small-scale fermentative hydrogen production system were 41.2 mLVh-1.L-1 and 35.1 mL/h-1.L-1, respectively. CONCLUSION: Therefore, the results indicate that the hydrogen production efficiency of the two-step process is higher than that of the one-step process.
author	Chen,Peng Wang,Yuxia Yan,Lei Wang,Yiqing Li,Suyue Yan,Xiaojuan Wang,Ningbo Liang,Ning Li,Hongyu
author_facet	Chen,Peng Wang,Yuxia Yan,Lei Wang,Yiqing Li,Suyue Yan,Xiaojuan Wang,Ningbo Liang,Ning Li,Hongyu
author_sort	Chen,Peng
title	Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
title_short	Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
title_full	Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
title_fullStr	Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
title_full_unstemmed	Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
title_sort	feasibility of biohydrogen production from industrial wastes using defined microbial co-culture
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100024
work_keys_str_mv	AT chenpeng feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT wangyuxia feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT yanlei feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT wangyiqing feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT lisuyue feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT yanxiaojuan feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT wangningbo feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT liangning feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture AT lihongyu feasibilityofbiohydrogenproductionfromindustrialwastesusingdefinedmicrobialcoculture
_version_	1718441533260169216

Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture

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