Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings

BACKGROUND: Ruta graveolens L. (R. graveolens) is a medicinal plant employed in non-traditional medicines that has various therapeutic properties, including anthelmintic, and vasodilatory actions, among others. We evaluated the trachea-relaxant effects of hydroalcoholic extract of R. graveolens agai...

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Autores principales:	Águila,Luis, Ruedlinger,Jenny, Mansilla,Karina, Ordenes,José, Salvatici,Raúl, Campos,Rui Ribeiro de, Romero,Fernando
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Ruta graveolens Trachea-relaxant Plant extract
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100028
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spelling	oai:scielo:S0716-976020150001000282016-02-16Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal ringsÁguila,LuisRuedlinger,JennyMansilla,KarinaOrdenes,JoséSalvatici,RaúlCampos,Rui Ribeiro deRomero,Fernando Ruta graveolens Trachea-relaxant Plant extract BACKGROUND: Ruta graveolens L. (R. graveolens) is a medicinal plant employed in non-traditional medicines that has various therapeutic properties, including anthelmintic, and vasodilatory actions, among others. We evaluated the trachea-relaxant effects of hydroalcoholic extract of R. graveolens against potassium chloride (KCl)- and carbachol-induced contraction of rat tracheal rings in an isolated organ bath. RESULTS: The results showed that the airway smooth muscle contraction induced by the depolarizing agent (KCl) and cholinergic agonist (carbachol) was markedly reduced by R. graveolens in a concentration-dependent manner, with maximum values of 109 ± 7.9 % and 118 ± 2.6 %, respectively (changes in tension expressed as positive percentages of change in proportion to maximum contraction), at the concentration of 45 μg/mL (half-maximal inhibitory concentration IC50: 35.5 μg/mL and 27.8 μg/mL for KCl- and carbachol-induced contraction, respectively). Additionally, the presence of R. graveolens produced rightward parallel displacement of carbachol dose-response curves and reduced over 35 % of the maximum smooth muscle contraction. CONCLUSIONS: The hydroalcoholic extract of R. graveolens exhibited relaxant activity on rat tracheal rings. The results suggest that the trachea-relaxant effect is mediated by a non-competitive antagonistic mechanism. More detailed studies are needed to identify the target of the inhibition, and to determine more precisely the pharmacological mechanisms involved in the observed biological effects.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100028en10.1186/S40659-015-0017-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ruta graveolens Trachea-relaxant Plant extract
spellingShingle	Ruta graveolens Trachea-relaxant Plant extract Águila,Luis Ruedlinger,Jenny Mansilla,Karina Ordenes,José Salvatici,Raúl Campos,Rui Ribeiro de Romero,Fernando Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
description	BACKGROUND: Ruta graveolens L. (R. graveolens) is a medicinal plant employed in non-traditional medicines that has various therapeutic properties, including anthelmintic, and vasodilatory actions, among others. We evaluated the trachea-relaxant effects of hydroalcoholic extract of R. graveolens against potassium chloride (KCl)- and carbachol-induced contraction of rat tracheal rings in an isolated organ bath. RESULTS: The results showed that the airway smooth muscle contraction induced by the depolarizing agent (KCl) and cholinergic agonist (carbachol) was markedly reduced by R. graveolens in a concentration-dependent manner, with maximum values of 109 ± 7.9 % and 118 ± 2.6 %, respectively (changes in tension expressed as positive percentages of change in proportion to maximum contraction), at the concentration of 45 μg/mL (half-maximal inhibitory concentration IC50: 35.5 μg/mL and 27.8 μg/mL for KCl- and carbachol-induced contraction, respectively). Additionally, the presence of R. graveolens produced rightward parallel displacement of carbachol dose-response curves and reduced over 35 % of the maximum smooth muscle contraction. CONCLUSIONS: The hydroalcoholic extract of R. graveolens exhibited relaxant activity on rat tracheal rings. The results suggest that the trachea-relaxant effect is mediated by a non-competitive antagonistic mechanism. More detailed studies are needed to identify the target of the inhibition, and to determine more precisely the pharmacological mechanisms involved in the observed biological effects.
author	Águila,Luis Ruedlinger,Jenny Mansilla,Karina Ordenes,José Salvatici,Raúl Campos,Rui Ribeiro de Romero,Fernando
author_facet	Águila,Luis Ruedlinger,Jenny Mansilla,Karina Ordenes,José Salvatici,Raúl Campos,Rui Ribeiro de Romero,Fernando
author_sort	Águila,Luis
title	Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
title_short	Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
title_full	Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
title_fullStr	Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
title_full_unstemmed	Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings
title_sort	relaxant effects of a hydroalcoholic extract of ruta graveolens on isolated rat tracheal rings
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100028
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Relaxant effects of a hydroalcoholic extract of Ruta graveolens on isolated rat tracheal rings

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