Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee
BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecul...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad de Biología de Chile
2015
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100031 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0716-97602015000100031 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0716-976020150001000312016-02-16Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzeeGonzález-Alvarez,RafaelGarza-Rodriguez,María de LourdesDelgado-Enciso,IvánTreviño-Alvarado,Victor ManuelCanales-Del-Castillo,RicardoMartínez-De-Villarreal,Laura EliaLugo-Trampe,ÁngelTejero,María ElizabethSchlabritz-Loutsevitch,Natalia E.Rocha-Pizaña,María Del RefugioCole,Shelley A.Reséndez-Pérez,DianaMoises-Alvarez,MarioComuzzie,Anthony G.Barrera-Saldaña,Hugo AlbertoGarza-Guajardo,RaquelBarboza-Quintana,OraliaRodríguez-Sánchez,Irám Pablo Retinoic acid receptor Responder protein Primate Chemerin TIG2 Tazarotene-induced gene 2 protein Gene expression BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we performed A RT-PCR from different tissues obtained during necropsies. Also we tested the hypotheses of positive evolution, purifying selection, and neutrality. And finally a phylogenetic analysis was made between primates RARRES2 protein. RESULTS: RARRES2 transcripts were present in liver, lung, adipose tissue, ovary, pancreas, heart, hypothalamus and pituitary tissues. Expression in kidney and leukocytes were not detectable in either species. It was determined that the studied genes are orthologous. CONCLUSIONS: RARRES2 evolution fits the hypothesis of purifying selection. Expression profiles of the RARRES2 gene are similar in baboons and chimpanzees and are also phylogenetically related.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100031en10.1186/S40659-015-0020-0 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Retinoic acid receptor Responder protein Primate Chemerin TIG2 Tazarotene-induced gene 2 protein Gene expression |
| spellingShingle |
Retinoic acid receptor Responder protein Primate Chemerin TIG2 Tazarotene-induced gene 2 protein Gene expression González-Alvarez,Rafael Garza-Rodriguez,María de Lourdes Delgado-Enciso,Iván Treviño-Alvarado,Victor Manuel Canales-Del-Castillo,Ricardo Martínez-De-Villarreal,Laura Elia Lugo-Trampe,Ángel Tejero,María Elizabeth Schlabritz-Loutsevitch,Natalia E. Rocha-Pizaña,María Del Refugio Cole,Shelley A. Reséndez-Pérez,Diana Moises-Alvarez,Mario Comuzzie,Anthony G. Barrera-Saldaña,Hugo Alberto Garza-Guajardo,Raquel Barboza-Quintana,Oralia Rodríguez-Sánchez,Irám Pablo Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| description |
BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we performed A RT-PCR from different tissues obtained during necropsies. Also we tested the hypotheses of positive evolution, purifying selection, and neutrality. And finally a phylogenetic analysis was made between primates RARRES2 protein. RESULTS: RARRES2 transcripts were present in liver, lung, adipose tissue, ovary, pancreas, heart, hypothalamus and pituitary tissues. Expression in kidney and leukocytes were not detectable in either species. It was determined that the studied genes are orthologous. CONCLUSIONS: RARRES2 evolution fits the hypothesis of purifying selection. Expression profiles of the RARRES2 gene are similar in baboons and chimpanzees and are also phylogenetically related. |
| author |
González-Alvarez,Rafael Garza-Rodriguez,María de Lourdes Delgado-Enciso,Iván Treviño-Alvarado,Victor Manuel Canales-Del-Castillo,Ricardo Martínez-De-Villarreal,Laura Elia Lugo-Trampe,Ángel Tejero,María Elizabeth Schlabritz-Loutsevitch,Natalia E. Rocha-Pizaña,María Del Refugio Cole,Shelley A. Reséndez-Pérez,Diana Moises-Alvarez,Mario Comuzzie,Anthony G. Barrera-Saldaña,Hugo Alberto Garza-Guajardo,Raquel Barboza-Quintana,Oralia Rodríguez-Sánchez,Irám Pablo |
| author_facet |
González-Alvarez,Rafael Garza-Rodriguez,María de Lourdes Delgado-Enciso,Iván Treviño-Alvarado,Victor Manuel Canales-Del-Castillo,Ricardo Martínez-De-Villarreal,Laura Elia Lugo-Trampe,Ángel Tejero,María Elizabeth Schlabritz-Loutsevitch,Natalia E. Rocha-Pizaña,María Del Refugio Cole,Shelley A. Reséndez-Pérez,Diana Moises-Alvarez,Mario Comuzzie,Anthony G. Barrera-Saldaña,Hugo Alberto Garza-Guajardo,Raquel Barboza-Quintana,Oralia Rodríguez-Sánchez,Irám Pablo |
| author_sort |
González-Alvarez,Rafael |
| title |
Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| title_short |
Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| title_full |
Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| title_fullStr |
Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| title_full_unstemmed |
Molecular evolution and expression profile of the chemerine encoding gene RARRES2 in baboon and chimpanzee |
| title_sort |
molecular evolution and expression profile of the chemerine encoding gene rarres2 in baboon and chimpanzee |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2015 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100031 |
| work_keys_str_mv |
AT gonzalezalvarezrafael molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT garzarodriguezmariadelourdes molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT delgadoencisoivan molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT trevinoalvaradovictormanuel molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT canalesdelcastilloricardo molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT martinezdevillarreallauraelia molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT lugotrampeangel molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT tejeromariaelizabeth molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT schlabritzloutsevitchnataliae molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT rochapizanamariadelrefugio molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT coleshelleya molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT resendezperezdiana molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT moisesalvarezmario molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT comuzzieanthonyg molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT barrerasaldanahugoalberto molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT garzaguajardoraquel molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT barbozaquintanaoralia molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee AT rodriguezsanchezirampablo molecularevolutionandexpressionprofileofthechemerineencodinggenerarres2inbaboonandchimpanzee |
| _version_ |
1718441534973542400 |