stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells

BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Berrocal,Liliana, Fuentes,Juan A, Nicole Trombert,A, Jofré,Matías R, Villagra,Nicolás A, Valenzuela,Luis M, Mora,Guido C
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Salmonella Typhi stg Fimbriae Adherence Association bacteria-eukaryotic cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100034
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602015000100034
record_format	dspace
spelling	oai:scielo:S0716-976020150001000342016-02-16stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cellsBerrocal,LilianaFuentes,Juan ANicole Trombert,AJofré,Matías RVillagra,Nicolás AValenzuela,Luis MMora,Guido C Salmonella Typhi stg Fimbriae Adherence Association bacteria-eukaryotic cells BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and mononuclear cells directly taken from human peripheral blood. RESULTS: We compared S. Typhi STH2370 WT, a Chilean clinical strain, and the S. Typhi STH2370 Astg mutant with respect to association and invasion using epithelial and macrophage-like cells. We observed that deletion of stg operon reduced the association and invasion of S. Typhi, in both cellular types. The presence of the cloned stg operon restored the WT phenotype in all the cases. Moreover, we compared Salmonella enterica sv. Typhimurium 14028s (S. Typhimurium, a serovar lacking stg operon) and S. Typhimurium heterologously expressing S. Typhi stg. We found that the latter presents an increased cell disruption of polarized epithelial cells and an increased association in both epithelial and macrophage-like cells. CONCLUSIONS: S. Typhi stg operon encodes a functional adhesin that participates in the interaction bacteria-eukary-otic cells, including epithelial cells and macrophages-like cells. The phenotypes associated to stg operon include increased association and consequent invasion in bacteria-eukaryotic cells, and cell disruption.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100034en10.1186/S40659-015-0024-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Salmonella Typhi stg Fimbriae Adherence Association bacteria-eukaryotic cells
spellingShingle	Salmonella Typhi stg Fimbriae Adherence Association bacteria-eukaryotic cells Berrocal,Liliana Fuentes,Juan A Nicole Trombert,A Jofré,Matías R Villagra,Nicolás A Valenzuela,Luis M Mora,Guido C stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
description	BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and mononuclear cells directly taken from human peripheral blood. RESULTS: We compared S. Typhi STH2370 WT, a Chilean clinical strain, and the S. Typhi STH2370 Astg mutant with respect to association and invasion using epithelial and macrophage-like cells. We observed that deletion of stg operon reduced the association and invasion of S. Typhi, in both cellular types. The presence of the cloned stg operon restored the WT phenotype in all the cases. Moreover, we compared Salmonella enterica sv. Typhimurium 14028s (S. Typhimurium, a serovar lacking stg operon) and S. Typhimurium heterologously expressing S. Typhi stg. We found that the latter presents an increased cell disruption of polarized epithelial cells and an increased association in both epithelial and macrophage-like cells. CONCLUSIONS: S. Typhi stg operon encodes a functional adhesin that participates in the interaction bacteria-eukary-otic cells, including epithelial cells and macrophages-like cells. The phenotypes associated to stg operon include increased association and consequent invasion in bacteria-eukaryotic cells, and cell disruption.
author	Berrocal,Liliana Fuentes,Juan A Nicole Trombert,A Jofré,Matías R Villagra,Nicolás A Valenzuela,Luis M Mora,Guido C
author_facet	Berrocal,Liliana Fuentes,Juan A Nicole Trombert,A Jofré,Matías R Villagra,Nicolás A Valenzuela,Luis M Mora,Guido C
author_sort	Berrocal,Liliana
title	stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
title_short	stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
title_full	stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
title_fullStr	stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
title_full_unstemmed	stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
title_sort	stg fimbrial operon from s. typhi sth2370 contributes to association and cell disruption of epithelial and macrophage-like cells
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100034
work_keys_str_mv	AT berrocalliliana stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT fuentesjuana stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT nicoletromberta stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT jofrematiasr stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT villagranicolasa stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT valenzuelaluism stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells AT moraguidoc stgfimbrialoperonfromstyphisth2370contributestoassociationandcelldisruptionofepithelialandmacrophagelikecells
_version_	1718441535810306048

stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells

Ejemplares similares