Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells

BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULT...

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Autores principales:	Guo,Jian-Ru, Chen,Qian-Qian, Lam,Christopher Wai-Kei, Zhang,Wei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Karanjin Cell cycle Apoptosis Cancer therapy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040
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spelling	oai:scielo:S0716-976020150001000402016-02-16Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cellsGuo,Jian-RuChen,Qian-QianLam,Christopher Wai-KeiZhang,Wei Karanjin Cell cycle Apoptosis Cancer therapy BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. CONCLUSION: Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040en10.1186/S40659-015-0031-X
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Karanjin Cell cycle Apoptosis Cancer therapy
spellingShingle	Karanjin Cell cycle Apoptosis Cancer therapy Guo,Jian-Ru Chen,Qian-Qian Lam,Christopher Wai-Kei Zhang,Wei Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
description	BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. CONCLUSION: Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy.
author	Guo,Jian-Ru Chen,Qian-Qian Lam,Christopher Wai-Kei Zhang,Wei
author_facet	Guo,Jian-Ru Chen,Qian-Qian Lam,Christopher Wai-Kei Zhang,Wei
author_sort	Guo,Jian-Ru
title	Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
title_short	Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
title_full	Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
title_fullStr	Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
title_full_unstemmed	Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
title_sort	effects of karanjin on cell cycle arrest and apoptosis in human a549, hepg2 and hl-60 cancer cells
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040
work_keys_str_mv	AT guojianru effectsofkaranjinoncellcyclearrestandapoptosisinhumana549hepg2andhl60cancercells AT chenqianqian effectsofkaranjinoncellcyclearrestandapoptosisinhumana549hepg2andhl60cancercells AT lamchristopherwaikei effectsofkaranjinoncellcyclearrestandapoptosisinhumana549hepg2andhl60cancercells AT zhangwei effectsofkaranjinoncellcyclearrestandapoptosisinhumana549hepg2andhl60cancercells
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Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells

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