Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

BACKGROUND: Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To tes...

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Autores principales:	Takeyama,Masayuki, Yoneda,Masahiko, Gosho,Masahiko, Iwaki,Masayoshi, Zako,Masahiro
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Age-related macular degeneration (AMD) Hypothermia Pigment epithelium-derived factor (PEDF) Retinal pigment epithelium (RPE) Vascular endothelial growth factor (VEGF) Vitrectomy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100042
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spelling	oai:scielo:S0716-976020150001000422016-02-16Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermiaTakeyama,MasayukiYoneda,MasahikoGosho,MasahikoIwaki,MasayoshiZako,Masahiro Age-related macular degeneration (AMD) Hypothermia Pigment epithelium-derived factor (PEDF) Retinal pigment epithelium (RPE) Vascular endothelial growth factor (VEGF) Vitrectomy BACKGROUND: Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogen-esis in retinal pigment epithelium (RPE). RESULTS: We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium-derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased. CONCLUSIONS: RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100042en10.1186/S40659-015-0034-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Age-related macular degeneration (AMD) Hypothermia Pigment epithelium-derived factor (PEDF) Retinal pigment epithelium (RPE) Vascular endothelial growth factor (VEGF) Vitrectomy
spellingShingle	Age-related macular degeneration (AMD) Hypothermia Pigment epithelium-derived factor (PEDF) Retinal pigment epithelium (RPE) Vascular endothelial growth factor (VEGF) Vitrectomy Takeyama,Masayuki Yoneda,Masahiko Gosho,Masahiko Iwaki,Masayoshi Zako,Masahiro Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
description	BACKGROUND: Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogen-esis in retinal pigment epithelium (RPE). RESULTS: We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium-derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased. CONCLUSIONS: RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.
author	Takeyama,Masayuki Yoneda,Masahiko Gosho,Masahiko Iwaki,Masayoshi Zako,Masahiro
author_facet	Takeyama,Masayuki Yoneda,Masahiko Gosho,Masahiko Iwaki,Masayoshi Zako,Masahiro
author_sort	Takeyama,Masayuki
title	Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
title_short	Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
title_full	Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
title_fullStr	Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
title_full_unstemmed	Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
title_sort	decreased vegf-a and sustained pedf expression in a human retinal pigment epithelium cell line cultured under hypothermia
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100042
work_keys_str_mv	AT takeyamamasayuki decreasedvegfaandsustainedpedfexpressioninahumanretinalpigmentepitheliumcelllineculturedunderhypothermia AT yonedamasahiko decreasedvegfaandsustainedpedfexpressioninahumanretinalpigmentepitheliumcelllineculturedunderhypothermia AT goshomasahiko decreasedvegfaandsustainedpedfexpressioninahumanretinalpigmentepitheliumcelllineculturedunderhypothermia AT iwakimasayoshi decreasedvegfaandsustainedpedfexpressioninahumanretinalpigmentepitheliumcelllineculturedunderhypothermia AT zakomasahiro decreasedvegfaandsustainedpedfexpressioninahumanretinalpigmentepitheliumcelllineculturedunderhypothermia
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Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

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