Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E

BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanism...

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Autores principales:	Santana,Andréia T, Guelfi,Marieli, Medeiros,Hyllana C D, Tavares,Marco A, Bizerra,Paulo F V, Mingatto,Fábio E
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100043
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spelling	oai:scielo:S0716-976020150001000432016-02-16Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin ESantana,Andréia TGuelfi,MarieliMedeiros,Hyllana C DTavares,Marco ABizerra,Paulo F VMingatto,Fábio E Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/ saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. RESULTS: Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididy-mal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogen-ate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxida-tion indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). CONCLUSIONS: This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100043en10.1186/S40659-015-0026-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E
spellingShingle	Gossypol Fertility Oxidative stress Bioenergetics Antioxidant system Vitamin E Santana,Andréia T Guelfi,Marieli Medeiros,Hyllana C D Tavares,Marco A Bizerra,Paulo F V Mingatto,Fábio E Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
description	BACKGROUND: Gossypol is a chemical present in the seeds of cotton plants (Gossypium sp.) that reduces fertility in farm animals. Vitamin E is an antioxidant and may help to protect cells and tissues against the deleterious effects of free radicals. The aim of this study was to evaluate the mechanisms of reproductive toxicity of gossypol in rats and the protective effects of vitamin E. Forty Wistar rats were used, divided into four experimental groups (n = 10): DMSO/ saline + corn oil; DMSO/saline + vitamin E; gossypol + corn oil; and gossypol + vitamin E. RESULTS: Fertility was significantly reduced in male rats treated with gossypol in that a significant decrease in epididy-mal sperm count was observed (P < 0.05) and the number of offspring was significantly reduced in females mated with them (P < 0.05). This dysfunction was prevented by vitamin E. Gossypol caused a significant increase in the activity of the enzymes glutathione peroxidase (P < 0.01) and glutathione reductase (P < 0.01), but vitamin E did not reduce the enzyme activities (P > 0.05). The levels of reduced glutathione and pyridine nucleotides in testis homogen-ate were significantly reduced by gossypol (P < 0.05 and P < 0.01, respectively) and this reduction was accompanied by increased levels of oxidized glutathione (P < 0.05). Vitamin E showed a preventive effect on the changes in the levels of these substances. Gossypol significantly increased the levels of malondialdehyde (P < 0.01), a lipid peroxida-tion indicator, whereas treatment with vitamin E inhibited the action of the gossypol. Vitamin E prevented a decrease in mitochondrial ATP induced by gossypol (P < 0.05). CONCLUSIONS: This study suggests that the reproductive dysfunction caused by gossypol may be related to oxidative stress and mitochondrial bioenergetic damage and that treatment with vitamin E can prevent the infertility caused by the toxin.
author	Santana,Andréia T Guelfi,Marieli Medeiros,Hyllana C D Tavares,Marco A Bizerra,Paulo F V Mingatto,Fábio E
author_facet	Santana,Andréia T Guelfi,Marieli Medeiros,Hyllana C D Tavares,Marco A Bizerra,Paulo F V Mingatto,Fábio E
author_sort	Santana,Andréia T
title	Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
title_short	Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
title_full	Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
title_fullStr	Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
title_full_unstemmed	Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E
title_sort	mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin e
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100043
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Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E

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