Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway

BACKGROUND: The fetus is surrounded by the amniotic fluid (AF) contained by the amniotic sac of the pregnant female. The AF is directly conveyed to the fetus during pregnancy. Although AF has recently been reported as an untapped resource containing various substances, it remains unclear whether the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jang,Yongwoo, Kim,Eun-Kyung, Shim,Won-Sik, Song,Ki-Min, Kim,Sung Min
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Amniotic fluid Extracellular signal-regulated kinase (ERK) Glycogen synthase kinase-3 (GSK-3) Neural clustering Neurosphere Fetal development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100044
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602015000100044
record_format	dspace
spelling	oai:scielo:S0716-976020150001000442016-02-16Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathwayJang,YongwooKim,Eun-KyungShim,Won-SikSong,Ki-MinKim,Sung Min Amniotic fluid Extracellular signal-regulated kinase (ERK) Glycogen synthase kinase-3 (GSK-3) Neural clustering Neurosphere Fetal development BACKGROUND: The fetus is surrounded by the amniotic fluid (AF) contained by the amniotic sac of the pregnant female. The AF is directly conveyed to the fetus during pregnancy. Although AF has recently been reported as an untapped resource containing various substances, it remains unclear whether the AF could influence fetal neurodevelopment. RESULTS: We used AF that was extracted from embryos at 16 days in pregnant SD rat and exposed the AF to the neural cells derived from the embryos of same rat. We found that the treatment of AF to cortical neurons increased the phosphorylation in ERK1/2 that is necessary for fetal neurodevelopment, which was inhibited by the treatment of MEK inhibitors. Moreover, we found the subsequent inhibition of glycogen synthase kinase-3 (GSK-3), which is an important determinant of cell fate in neural cells. Indeed, AF increased the neural clustering of cortical neurons, which revealed that the clustered cells were proliferating neural progenitor cells. Accordingly, we confirmed the ability of AF to increase the neural progenitor cells through neurosphere formation. Furthermore, we showed that the ERK/GSK-3 pathway was involved in AF-mediated neurosphere enlargement. CONCLUSIONS: Although the placenta mainly supplies oxygenated blood, nutrient substances for fetal development, these findings further suggest that circulating-AF into the fetus could affect fetal neurodevelopment via MAP kinases-derived GSK-3 pathway during pregnancy. Moreover, we suggest that AF could be utilized as a valuable resource in the field of regenerative medicine.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100044en10.1186/S40659-015-0029-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Amniotic fluid Extracellular signal-regulated kinase (ERK) Glycogen synthase kinase-3 (GSK-3) Neural clustering Neurosphere Fetal development
spellingShingle	Amniotic fluid Extracellular signal-regulated kinase (ERK) Glycogen synthase kinase-3 (GSK-3) Neural clustering Neurosphere Fetal development Jang,Yongwoo Kim,Eun-Kyung Shim,Won-Sik Song,Ki-Min Kim,Sung Min Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
description	BACKGROUND: The fetus is surrounded by the amniotic fluid (AF) contained by the amniotic sac of the pregnant female. The AF is directly conveyed to the fetus during pregnancy. Although AF has recently been reported as an untapped resource containing various substances, it remains unclear whether the AF could influence fetal neurodevelopment. RESULTS: We used AF that was extracted from embryos at 16 days in pregnant SD rat and exposed the AF to the neural cells derived from the embryos of same rat. We found that the treatment of AF to cortical neurons increased the phosphorylation in ERK1/2 that is necessary for fetal neurodevelopment, which was inhibited by the treatment of MEK inhibitors. Moreover, we found the subsequent inhibition of glycogen synthase kinase-3 (GSK-3), which is an important determinant of cell fate in neural cells. Indeed, AF increased the neural clustering of cortical neurons, which revealed that the clustered cells were proliferating neural progenitor cells. Accordingly, we confirmed the ability of AF to increase the neural progenitor cells through neurosphere formation. Furthermore, we showed that the ERK/GSK-3 pathway was involved in AF-mediated neurosphere enlargement. CONCLUSIONS: Although the placenta mainly supplies oxygenated blood, nutrient substances for fetal development, these findings further suggest that circulating-AF into the fetus could affect fetal neurodevelopment via MAP kinases-derived GSK-3 pathway during pregnancy. Moreover, we suggest that AF could be utilized as a valuable resource in the field of regenerative medicine.
author	Jang,Yongwoo Kim,Eun-Kyung Shim,Won-Sik Song,Ki-Min Kim,Sung Min
author_facet	Jang,Yongwoo Kim,Eun-Kyung Shim,Won-Sik Song,Ki-Min Kim,Sung Min
author_sort	Jang,Yongwoo
title	Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
title_short	Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
title_full	Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
title_fullStr	Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
title_full_unstemmed	Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway
title_sort	amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the erk/gsk-3 pathway
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100044
work_keys_str_mv	AT jangyongwoo amnioticfluidexertsaneurotrophicinfluenceonfetalneurodevelopmentviatheerkgsk3pathway AT kimeunkyung amnioticfluidexertsaneurotrophicinfluenceonfetalneurodevelopmentviatheerkgsk3pathway AT shimwonsik amnioticfluidexertsaneurotrophicinfluenceonfetalneurodevelopmentviatheerkgsk3pathway AT songkimin amnioticfluidexertsaneurotrophicinfluenceonfetalneurodevelopmentviatheerkgsk3pathway AT kimsungmin amnioticfluidexertsaneurotrophicinfluenceonfetalneurodevelopmentviatheerkgsk3pathway
_version_	1718441538623635456

Amniotic fluid exerts a neurotrophic influence on fetal neurodevelopment via the ERK/GSK-3 pathway

Ejemplares similares