Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells

BACKGROUND: The collective cell migration of stratified epithelial cells is considered to be an important phenomenon in wound healing, development, and cancer invasion; however, little is known about the mechanisms involved. Furthermore, whereas Rho family proteins, including RhoA, play important ro...

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Autores principales:	Mikami,Taro, Yoshida,Keiichiro, Sawada,Hajime, Esaki,Michiyo, Yasumura,Kazunori, Ono,Michio
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Collective cell migration Stratified epithelium TE-10 cells Rho-associated coiled coil-containing protein kinase siRNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100048
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spelling	oai:scielo:S0716-976020150001000482016-02-16Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cellsMikami,TaroYoshida,KeiichiroSawada,HajimeEsaki,MichiyoYasumura,KazunoriOno,Michio Collective cell migration Stratified epithelium TE-10 cells Rho-associated coiled coil-containing protein kinase siRNA BACKGROUND: The collective cell migration of stratified epithelial cells is considered to be an important phenomenon in wound healing, development, and cancer invasion; however, little is known about the mechanisms involved. Furthermore, whereas Rho family proteins, including RhoA, play important roles in cell migration, the exact role of Rho-associated coiled coil-containing protein kinases (ROCKs) in cell migration is controversial and might be cell-type dependent. Here, we report the development of a novel modified scratch assay that was used to observe the collective cell migration of stratified TE-10 cells derived from a human esophageal cancer specimen. RESULTS: Desmosomes were found between the TE-10 cells and microvilli of the surface of the cell sheet. The leading edge of cells in the cell sheet formed a simple layer and moved forward regularly; these rows were followed by the stratified epithelium. ROCK inhibitors and ROCK small interfering RNAs (siRNAs) disturbed not only the collective migration of the leading edge of this cell sheet, but also the stratified layer in the rear. In contrast, RhoA siRNA treatment resulted in more rapid migration of the leading rows and disturbed movement of the stratified portion. CONCLUSIONS: The data presented in this study suggest that ROCKs play an important role in mediating the collective migration of TE-10 cell sheets. In addition, differences between the effects of siRNAs targeting either RhoA or ROCKs suggested that distinct mechanisms regulate the collective cell migration in the simple epithelium of the wound edge versus the stratified layer of the epithelium.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100048en10.1186/S40659-015-0039-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Collective cell migration Stratified epithelium TE-10 cells Rho-associated coiled coil-containing protein kinase siRNA
spellingShingle	Collective cell migration Stratified epithelium TE-10 cells Rho-associated coiled coil-containing protein kinase siRNA Mikami,Taro Yoshida,Keiichiro Sawada,Hajime Esaki,Michiyo Yasumura,Kazunori Ono,Michio Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
description	BACKGROUND: The collective cell migration of stratified epithelial cells is considered to be an important phenomenon in wound healing, development, and cancer invasion; however, little is known about the mechanisms involved. Furthermore, whereas Rho family proteins, including RhoA, play important roles in cell migration, the exact role of Rho-associated coiled coil-containing protein kinases (ROCKs) in cell migration is controversial and might be cell-type dependent. Here, we report the development of a novel modified scratch assay that was used to observe the collective cell migration of stratified TE-10 cells derived from a human esophageal cancer specimen. RESULTS: Desmosomes were found between the TE-10 cells and microvilli of the surface of the cell sheet. The leading edge of cells in the cell sheet formed a simple layer and moved forward regularly; these rows were followed by the stratified epithelium. ROCK inhibitors and ROCK small interfering RNAs (siRNAs) disturbed not only the collective migration of the leading edge of this cell sheet, but also the stratified layer in the rear. In contrast, RhoA siRNA treatment resulted in more rapid migration of the leading rows and disturbed movement of the stratified portion. CONCLUSIONS: The data presented in this study suggest that ROCKs play an important role in mediating the collective migration of TE-10 cell sheets. In addition, differences between the effects of siRNAs targeting either RhoA or ROCKs suggested that distinct mechanisms regulate the collective cell migration in the simple epithelium of the wound edge versus the stratified layer of the epithelium.
author	Mikami,Taro Yoshida,Keiichiro Sawada,Hajime Esaki,Michiyo Yasumura,Kazunori Ono,Michio
author_facet	Mikami,Taro Yoshida,Keiichiro Sawada,Hajime Esaki,Michiyo Yasumura,Kazunori Ono,Michio
author_sort	Mikami,Taro
title	Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
title_short	Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
title_full	Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
title_fullStr	Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
title_full_unstemmed	Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells
title_sort	inhibition of rho-associated kinases disturbs the collective cell migration of stratified te-10 cells
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100048
work_keys_str_mv	AT mikamitaro inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells AT yoshidakeiichiro inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells AT sawadahajime inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells AT esakimichiyo inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells AT yasumurakazunori inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells AT onomichio inhibitionofrhoassociatedkinasesdisturbsthecollectivecellmigrationofstratifiedte10cells
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Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells

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