Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil

BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylatio...

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Autores principales:	Almeida,Fabiana Greyce Oliveira, Aquino,Priscila Ferreira de, Souza,Afonso Duarte Leäo de, Souza,Antonia Queiroz Lima de, Vinhote,Sonia do Carmo, Mac-Cormick,Thais Messias, Silva,Marcelo Soares da Mota, Chalub,Sidney Raimundo Silva, Fischer,Juliana de Saldanha da Gama, Carvalho,Paulo Costa, Carvalho,Maria da Gloria da Costa
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Methylation Colorectal cancer Amazon Epigenetic
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050
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spelling	oai:scielo:S0716-976020150001000502016-02-16Colorectal cancer DNA methylation patterns from patients in Manaus, BrazilAlmeida,Fabiana Greyce OliveiraAquino,Priscila Ferreira deSouza,Afonso Duarte Leäo deSouza,Antonia Queiroz Lima deVinhote,Sonia do CarmoMac-Cormick,Thais MessiasSilva,Marcelo Soares da MotaChalub,Sidney Raimundo SilvaFischer,Juliana de Saldanha da GamaCarvalho,Paulo CostaCarvalho,Maria da Gloria da Costa Methylation Colorectal cancer Amazon Epigenetic BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050en10.1186/S40659-015-0042-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Methylation Colorectal cancer Amazon Epigenetic
spellingShingle	Methylation Colorectal cancer Amazon Epigenetic Almeida,Fabiana Greyce Oliveira Aquino,Priscila Ferreira de Souza,Afonso Duarte Leäo de Souza,Antonia Queiroz Lima de Vinhote,Sonia do Carmo Mac-Cormick,Thais Messias Silva,Marcelo Soares da Mota Chalub,Sidney Raimundo Silva Fischer,Juliana de Saldanha da Gama Carvalho,Paulo Costa Carvalho,Maria da Gloria da Costa Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
description	BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively. CONCLUSIONS: Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations.
author	Almeida,Fabiana Greyce Oliveira Aquino,Priscila Ferreira de Souza,Afonso Duarte Leäo de Souza,Antonia Queiroz Lima de Vinhote,Sonia do Carmo Mac-Cormick,Thais Messias Silva,Marcelo Soares da Mota Chalub,Sidney Raimundo Silva Fischer,Juliana de Saldanha da Gama Carvalho,Paulo Costa Carvalho,Maria da Gloria da Costa
author_facet	Almeida,Fabiana Greyce Oliveira Aquino,Priscila Ferreira de Souza,Afonso Duarte Leäo de Souza,Antonia Queiroz Lima de Vinhote,Sonia do Carmo Mac-Cormick,Thais Messias Silva,Marcelo Soares da Mota Chalub,Sidney Raimundo Silva Fischer,Juliana de Saldanha da Gama Carvalho,Paulo Costa Carvalho,Maria da Gloria da Costa
author_sort	Almeida,Fabiana Greyce Oliveira
title	Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
title_short	Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
title_full	Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
title_fullStr	Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
title_full_unstemmed	Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
title_sort	colorectal cancer dna methylation patterns from patients in manaus, brazil
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050
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Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil

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