Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption

BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic...

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Autores principales:	Mamun,Md. Abdullah Al, Islam,Kamrul, Alam,Md. Jahangir, Khatun,Amina, Masihul Alam,M, Al-Bari,Md. Abdul Alim
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Bone resorption Osteoclast differentiation Pit formation TRAP positive cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051
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spelling	oai:scielo:S0716-976020150001000512016-02-16Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorptionMamun,Md. Abdullah AlIslam,KamrulAlam,Md. JahangirKhatun,AminaMasihul Alam,MAl-Bari,Md. Abdul AlimAlam,Md. Jahangir Bone resorption Osteoclast differentiation Pit formation TRAP positive cells BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive multinucleated cells and by measuring both TRAP activities. RESULTS: The TPF significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in primary osteoclastic cells. The TPF also decreased the expression of mRNAs related to osteoclast differentiation, including Trap, Cathepsin K, Mmp-9, and Mmp-13 in primary osteoclastic cells. The treatment of primary osteoclastic cells with the TPF decreased Cathepsin K, Mmp-9, and Mmp-13 proteins expression in primary osteoclastic cells. CONCLUSION: These results indicated that TPF inhibit osteoclastogenesis and pits formation activities. Our results suggest that the TPF could be a potential anti-bone resorptic agent to treat patients with bone loss-associated diseases such as osteoporosis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051en10.1186/S40659-015-0043-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bone resorption Osteoclast differentiation Pit formation TRAP positive cells
spellingShingle	Bone resorption Osteoclast differentiation Pit formation TRAP positive cells Mamun,Md. Abdullah Al Islam,Kamrul Alam,Md. Jahangir Khatun,Amina Masihul Alam,M Al-Bari,Md. Abdul Alim Alam,Md. Jahangir Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
description	BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive multinucleated cells and by measuring both TRAP activities. RESULTS: The TPF significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in primary osteoclastic cells. The TPF also decreased the expression of mRNAs related to osteoclast differentiation, including Trap, Cathepsin K, Mmp-9, and Mmp-13 in primary osteoclastic cells. The treatment of primary osteoclastic cells with the TPF decreased Cathepsin K, Mmp-9, and Mmp-13 proteins expression in primary osteoclastic cells. CONCLUSION: These results indicated that TPF inhibit osteoclastogenesis and pits formation activities. Our results suggest that the TPF could be a potential anti-bone resorptic agent to treat patients with bone loss-associated diseases such as osteoporosis.
author	Mamun,Md. Abdullah Al Islam,Kamrul Alam,Md. Jahangir Khatun,Amina Masihul Alam,M Al-Bari,Md. Abdul Alim Alam,Md. Jahangir
author_facet	Mamun,Md. Abdullah Al Islam,Kamrul Alam,Md. Jahangir Khatun,Amina Masihul Alam,M Al-Bari,Md. Abdul Alim Alam,Md. Jahangir
author_sort	Mamun,Md. Abdullah Al
title	Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
title_short	Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
title_full	Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
title_fullStr	Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
title_full_unstemmed	Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption
title_sort	flavonoids isolated from tridax procumbens (tpf) inhibit osteoclasts differentiation and bone resorption
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051
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Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption

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