Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats

BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP...

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Autores principales:	Ahmed,Rasha R., Mahmoud,Ayman, Ahmed,Osama M., Metwalli,Ali, Ebaid,Hossam
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Diabetic model Whey protein Heat shock protein-72 Keratin16 Wound healing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100054
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spelling	oai:scielo:S0716-976020150001000542016-02-16Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic ratsAhmed,Rasha R.Mahmoud,AymanAhmed,Osama M.Metwalli,AliEbaid,Hossam Diabetic model Whey protein Heat shock protein-72 Keratin16 Wound healing BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats. CONCLUSION: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100054en10.1186/s40659-015-0044-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diabetic model Whey protein Heat shock protein-72 Keratin16 Wound healing
spellingShingle	Diabetic model Whey protein Heat shock protein-72 Keratin16 Wound healing Ahmed,Rasha R. Mahmoud,Ayman Ahmed,Osama M. Metwalli,Ali Ebaid,Hossam Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
description	BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats. CONCLUSION: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.
author	Ahmed,Rasha R. Mahmoud,Ayman Ahmed,Osama M. Metwalli,Ali Ebaid,Hossam
author_facet	Ahmed,Rasha R. Mahmoud,Ayman Ahmed,Osama M. Metwalli,Ali Ebaid,Hossam
author_sort	Ahmed,Rasha R.
title	Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
title_short	Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
title_full	Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
title_fullStr	Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
title_full_unstemmed	Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
title_sort	up-regulation of hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100054
work_keys_str_mv	AT ahmedrashar upregulationofhsp72andkeratin16mediateswoundhealinginstreptozotocindiabeticrats AT mahmoudayman upregulationofhsp72andkeratin16mediateswoundhealinginstreptozotocindiabeticrats AT ahmedosamam upregulationofhsp72andkeratin16mediateswoundhealinginstreptozotocindiabeticrats AT metwalliali upregulationofhsp72andkeratin16mediateswoundhealinginstreptozotocindiabeticrats AT ebaidhossam upregulationofhsp72andkeratin16mediateswoundhealinginstreptozotocindiabeticrats
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Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats

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