Functional disruption of human leukocyte antigen II in human embryonic stem cell
BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exac...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Lenguaje: | English |
Publicado: |
Sociedad de Biología de Chile
2015
|
Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:scielo:S0716-97602015000100059 |
---|---|
record_format |
dspace |
spelling |
oai:scielo:S0716-976020150001000592016-02-16Functional disruption of human leukocyte antigen II in human embryonic stem cellChen,HaideLi,YangLin,XijuanCui,DiCui,ChunLi,HuiXiao,Lei hESCs CIITA TALENs Immune rejection BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA-/-hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from CIITA-/-hESCs expressed CD83 and CD86 but without the constitutive HLA II. Fibroblasts derived from CIITA-/-hESCs were powerless in IFN-γ inducible expression of HLA II CONCLUSION: We generated HLA II defected hESCs via deleting CIITA, a master regulator of constitutive and IFN-γ inducible expression of HLA II genes. CIITA-/-hESCs can differentiate into tissue cells with non-HLA II expression. It's promising that CIITA-/-hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors' CD4+ T cells, which are the main effector cells of cellular immunity in allograftinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059en10.1186/S40659-015-0051-6 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
topic |
hESCs CIITA TALENs Immune rejection |
spellingShingle |
hESCs CIITA TALENs Immune rejection Chen,Haide Li,Yang Lin,Xijuan Cui,Di Cui,Chun Li,Hui Xiao,Lei Functional disruption of human leukocyte antigen II in human embryonic stem cell |
description |
BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA-/-hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from CIITA-/-hESCs expressed CD83 and CD86 but without the constitutive HLA II. Fibroblasts derived from CIITA-/-hESCs were powerless in IFN-γ inducible expression of HLA II CONCLUSION: We generated HLA II defected hESCs via deleting CIITA, a master regulator of constitutive and IFN-γ inducible expression of HLA II genes. CIITA-/-hESCs can differentiate into tissue cells with non-HLA II expression. It's promising that CIITA-/-hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors' CD4+ T cells, which are the main effector cells of cellular immunity in allograft |
author |
Chen,Haide Li,Yang Lin,Xijuan Cui,Di Cui,Chun Li,Hui Xiao,Lei |
author_facet |
Chen,Haide Li,Yang Lin,Xijuan Cui,Di Cui,Chun Li,Hui Xiao,Lei |
author_sort |
Chen,Haide |
title |
Functional disruption of human leukocyte antigen II in human embryonic stem cell |
title_short |
Functional disruption of human leukocyte antigen II in human embryonic stem cell |
title_full |
Functional disruption of human leukocyte antigen II in human embryonic stem cell |
title_fullStr |
Functional disruption of human leukocyte antigen II in human embryonic stem cell |
title_full_unstemmed |
Functional disruption of human leukocyte antigen II in human embryonic stem cell |
title_sort |
functional disruption of human leukocyte antigen ii in human embryonic stem cell |
publisher |
Sociedad de Biología de Chile |
publishDate |
2015 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059 |
work_keys_str_mv |
AT chenhaide functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT liyang functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT linxijuan functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT cuidi functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT cuichun functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT lihui functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell AT xiaolei functionaldisruptionofhumanleukocyteantigeniiinhumanembryonicstemcell |
_version_ |
1718441542838910976 |