Tridax procumbens flavonoids promote osteoblast differentiation and bone formation

BACKGROUND: Tridaxprocumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains o...

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Autores principales:	Mamun,Md. Abdullah Al, Hosen,Mohammad Jakir, Islam,Kamrul, Khatun,Amina, Masihul Alam,M., Al-Bari,Md. Abdul Alim
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2015
Materias:	Alkaline phosphatase Bone formation Osteoblast differentiation Osteocalcin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100065
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spelling	oai:scielo:S0716-976020150001000652016-02-16Tridax procumbens flavonoids promote osteoblast differentiation and bone formationMamun,Md. Abdullah AlHosen,Mohammad JakirIslam,KamrulKhatun,AminaMasihul Alam,M.Al-Bari,Md. Abdul Alim Alkaline phosphatase Bone formation Osteoblast differentiation Osteocalcin BACKGROUND: Tridaxprocumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains of joints. TPFs have been reported to increase osteogenic functioning in mesenchymal stem cells. Our previous study showed that TPFs were significantly suppressed the RANKL-induced differentiation of osteoclasts and bone resorption. However, the effects of TPFs to promote osteoblasts differentiation and bone formation remain unclear. TPFs were isolated from Tridax procumbens and investigated for their effects on osteoblasts differentiation and bone formation by using primary mouse calvarial osteoblasts RESULTS: TPFs promoted osteoblast differentiation in a dose-dependent manner demonstrated by up-regulation of alkaline phosphatase and osteocalcin. TPFs also upregulated osteoblast differentiation related genes, including osteocalcin, osterix, and Runx2 in primary osteoblasts. TPFs treated primary osteoblast cells showed significant upregulation of bone morphogenetic proteins (BMPs) including Bmp-2, Bmp-4, and Bmp-7. Addition of noggin, a BMP specific-antagonist, inhibited TPFs induced upregulation of the osteocalcin, osterix, and Runx2 CONCLUSION: Our findings point towards the induction of osteoblast differentiation by TPFs and suggested that TPFs could be a potential anabolic agent to treat patients with bone loss-associated diseases such as osteoporosisinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100065en10.1186/S40659-015-0056-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Alkaline phosphatase Bone formation Osteoblast differentiation Osteocalcin
spellingShingle	Alkaline phosphatase Bone formation Osteoblast differentiation Osteocalcin Mamun,Md. Abdullah Al Hosen,Mohammad Jakir Islam,Kamrul Khatun,Amina Masihul Alam,M. Al-Bari,Md. Abdul Alim Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
description	BACKGROUND: Tridaxprocumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains of joints. TPFs have been reported to increase osteogenic functioning in mesenchymal stem cells. Our previous study showed that TPFs were significantly suppressed the RANKL-induced differentiation of osteoclasts and bone resorption. However, the effects of TPFs to promote osteoblasts differentiation and bone formation remain unclear. TPFs were isolated from Tridax procumbens and investigated for their effects on osteoblasts differentiation and bone formation by using primary mouse calvarial osteoblasts RESULTS: TPFs promoted osteoblast differentiation in a dose-dependent manner demonstrated by up-regulation of alkaline phosphatase and osteocalcin. TPFs also upregulated osteoblast differentiation related genes, including osteocalcin, osterix, and Runx2 in primary osteoblasts. TPFs treated primary osteoblast cells showed significant upregulation of bone morphogenetic proteins (BMPs) including Bmp-2, Bmp-4, and Bmp-7. Addition of noggin, a BMP specific-antagonist, inhibited TPFs induced upregulation of the osteocalcin, osterix, and Runx2 CONCLUSION: Our findings point towards the induction of osteoblast differentiation by TPFs and suggested that TPFs could be a potential anabolic agent to treat patients with bone loss-associated diseases such as osteoporosis
author	Mamun,Md. Abdullah Al Hosen,Mohammad Jakir Islam,Kamrul Khatun,Amina Masihul Alam,M. Al-Bari,Md. Abdul Alim
author_facet	Mamun,Md. Abdullah Al Hosen,Mohammad Jakir Islam,Kamrul Khatun,Amina Masihul Alam,M. Al-Bari,Md. Abdul Alim
author_sort	Mamun,Md. Abdullah Al
title	Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
title_short	Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
title_full	Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
title_fullStr	Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
title_full_unstemmed	Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
title_sort	tridax procumbens flavonoids promote osteoblast differentiation and bone formation
publisher	Sociedad de Biología de Chile
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100065
work_keys_str_mv	AT mamunmdabdullahal tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation AT hosenmohammadjakir tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation AT islamkamrul tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation AT khatunamina tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation AT masihulalamm tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation AT albarimdabdulalim tridaxprocumbensflavonoidspromoteosteoblastdifferentiationandboneformation
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Tridax procumbens flavonoids promote osteoblast differentiation and bone formation

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