Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line

BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to charac...

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Autores principales:	Buchegger,Kurt, Ili,Carmen, Riquelme,Ismael, Letelier,Pablo, Corvalán,Alejandro H., Brebi,Priscilla, Huang,Tim Hui-Ming, Roa,Juan Carlos
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Reprimo MDA-MB-231 Migration Invasion
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005
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spelling	oai:scielo:S0716-976020160001000052016-12-01Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell lineBuchegger,KurtIli,CarmenRiquelme,IsmaelLetelier,PabloCorvalán,Alejandro H.Brebi,PriscillaHuang,Tim Hui-MingRoa,Juan Carlos Reprimo MDA-MB-231 Migration Invasion BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERa(+) tumors showed higher methylation intensity than ERa(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. CONCLUSION: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005en10.1186/s40659-016-0066-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Reprimo MDA-MB-231 Migration Invasion
spellingShingle	Reprimo MDA-MB-231 Migration Invasion Buchegger,Kurt Ili,Carmen Riquelme,Ismael Letelier,Pablo Corvalán,Alejandro H. Brebi,Priscilla Huang,Tim Hui-Ming Roa,Juan Carlos Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
description	BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERa(+) tumors showed higher methylation intensity than ERa(-). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. CONCLUSION: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.
author	Buchegger,Kurt Ili,Carmen Riquelme,Ismael Letelier,Pablo Corvalán,Alejandro H. Brebi,Priscilla Huang,Tim Hui-Ming Roa,Juan Carlos
author_facet	Buchegger,Kurt Ili,Carmen Riquelme,Ismael Letelier,Pablo Corvalán,Alejandro H. Brebi,Priscilla Huang,Tim Hui-Ming Roa,Juan Carlos
author_sort	Buchegger,Kurt
title	Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
title_short	Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
title_full	Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
title_fullStr	Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
title_full_unstemmed	Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
title_sort	reprimo as a modulator of cell migration and invasion in the mda-mb-231 breast cancer cell line
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005
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Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line

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