Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons

Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons

BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs...

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Autores principales: Codocedo,Juan Francisco, Inestrosa,Nibaldo C.
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2016
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100009
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spelling oai:scielo:S0716-976020160001000092016-12-01Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neuronsCodocedo,Juan FranciscoInestrosa,Nibaldo C.BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs (miRNAs) are a family of small non-coding RNAs that control the gene expression of their targets through hybridization with complementary sequences in the 3' UTR, thereby inhibiting the translation of the target proteins. Several evidences indicate that the miRNAs are actively involved in the regulation of neuronal function RESULTS: In the present study, we examined whether Wnt-5a modulates the levels of miRNAs in hippocampal neurons. Using PCR arrays, we identified a set of miRNAs that respond to Wnt-5a treatment. One of the most affected miRNAs was miR-101b, which targets cyclooxygenase-2 (COX2), an inducible enzyme that converts arachidonic acid to prostanoids, and has been involved in the injury/inflammatory response, and more recently in neuronal plasticity. Consistent with the Wnt-5a regulation of miR-101b, this Wnt ligand regulates COX2 expression in a time-dependent manner in cultured hippocampal neurons CONCLUSION: The biological processes induced by Wnt-5a in hippocampal neurons, involve the regulation of several miRNAs including miR-101b, which has the capacity to regulate several targets, including COX-2 in the central nervous systeminfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100009en10.1186/s40659-016-0071-x
institution Scielo Chile
collection Scielo Chile
language English
description BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs (miRNAs) are a family of small non-coding RNAs that control the gene expression of their targets through hybridization with complementary sequences in the 3' UTR, thereby inhibiting the translation of the target proteins. Several evidences indicate that the miRNAs are actively involved in the regulation of neuronal function RESULTS: In the present study, we examined whether Wnt-5a modulates the levels of miRNAs in hippocampal neurons. Using PCR arrays, we identified a set of miRNAs that respond to Wnt-5a treatment. One of the most affected miRNAs was miR-101b, which targets cyclooxygenase-2 (COX2), an inducible enzyme that converts arachidonic acid to prostanoids, and has been involved in the injury/inflammatory response, and more recently in neuronal plasticity. Consistent with the Wnt-5a regulation of miR-101b, this Wnt ligand regulates COX2 expression in a time-dependent manner in cultured hippocampal neurons CONCLUSION: The biological processes induced by Wnt-5a in hippocampal neurons, involve the regulation of several miRNAs including miR-101b, which has the capacity to regulate several targets, including COX-2 in the central nervous system
author Codocedo,Juan Francisco
Inestrosa,Nibaldo C.
spellingShingle Codocedo,Juan Francisco
Inestrosa,Nibaldo C.
Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
author_facet Codocedo,Juan Francisco
Inestrosa,Nibaldo C.
author_sort Codocedo,Juan Francisco
title Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
title_short Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
title_full Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
title_fullStr Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
title_full_unstemmed Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons
title_sort wnt-5a-regulated mir-101b controls cox2 expression in hippocampal neurons
publisher Sociedad de Biología de Chile
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100009
work_keys_str_mv AT codocedojuanfrancisco wnt5aregulatedmir101bcontrolscox2expressioninhippocampalneurons
AT inestrosanibaldoc wnt5aregulatedmir101bcontrolscox2expressioninhippocampalneurons
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