Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis

BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats w...

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Autores principales:	Geng,Wenjia, Wei,Ribao, Liu,Shuwen, Tang,Li, Zhu,Hanyu, Chen,Pu, Wu,Jie, Zhang,Xueguang, Zhu,Fei, Yin,Zhong, Chen,Xiangmei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Shenhua Tablet Chronic anti-Thy-1 glomerulonephritis Mesangial cell proliferation Erk
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100017
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spelling	oai:scielo:S0716-976020160001000172016-12-01Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritisGeng,WenjiaWei,RibaoLiu,ShuwenTang,LiZhu,HanyuChen,PuWu,JieZhang,XueguangZhu,FeiYin,ZhongChen,Xiangmei Shenhua Tablet Chronic anti-Thy-1 glomerulonephritis Mesangial cell proliferation Erk BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100017en10.1186s40659-016-0078-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Shenhua Tablet Chronic anti-Thy-1 glomerulonephritis Mesangial cell proliferation Erk
spellingShingle	Shenhua Tablet Chronic anti-Thy-1 glomerulonephritis Mesangial cell proliferation Erk Geng,Wenjia Wei,Ribao Liu,Shuwen Tang,Li Zhu,Hanyu Chen,Pu Wu,Jie Zhang,Xueguang Zhu,Fei Yin,Zhong Chen,Xiangmei Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
description	BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological changes were thoroughly examined at the time point of 24 h. Meanwhile, the expression levels of p-Erk1/2, cyclin D1 and p21 at the renal cortex were also tested. The levels of urinary proteins and total cholesterol in the blood were significantly reduced in rats treated with any drug tested in this study. The level of triglyceride was significantly reduced in all three Shenhua Tablet-treated groups. Renal pathomorphological scores were significantly improved in groups of Irb, SHM and SHH. Mesangial cell proliferation was significantly inhibited in any drug-treated group. p-Erk1/2 and cyclin D1 were downregulated whereas p21 was upregulated in the renal cortex CONCLUSIONS: Our study indicated that Shenhua Tablet is able to inhibit the abnormal proliferation of mesangial cells and to prevent kidney damage, which is likely associated with downregulation of p-Erk1/2 and reduced activity of its downstream target-cyclin D1.
author	Geng,Wenjia Wei,Ribao Liu,Shuwen Tang,Li Zhu,Hanyu Chen,Pu Wu,Jie Zhang,Xueguang Zhu,Fei Yin,Zhong Chen,Xiangmei
author_facet	Geng,Wenjia Wei,Ribao Liu,Shuwen Tang,Li Zhu,Hanyu Chen,Pu Wu,Jie Zhang,Xueguang Zhu,Fei Yin,Zhong Chen,Xiangmei
author_sort	Geng,Wenjia
title	Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_short	Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_full	Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_fullStr	Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_full_unstemmed	Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis
title_sort	shenhua tablet inhibits mesangial cell proliferation in rats with chronic anti-thy-1 nephritis
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100017
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Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis

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