The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3

CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the ro...

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Autores principales:	Li,JinLong, Li,Hui, Zhang,ZhaoLi, Wang,NianYue, Zhang,Yong Chen
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Trichosanthin Interleukin IL-2 Prostate cancer cells PC3 Tumor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100021
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spelling	oai:scielo:S0716-976020160001000212016-12-01The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3Li,JinLongLi,HuiZhang,ZhaoLiWang,NianYueZhang,Yong Chen Trichosanthin Interleukin IL-2 Prostate cancer cells PC3 Tumor CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the role of trichosanthin on prostate cancer cells PC3. MATERIALS AND METHODS: Trichosanthin was expressed in BL21 strain and purified by affinity chromatography. MTT assay was designed to determine the effect of trichosanthin on growth of PC3 cells at doses of 10, 20, 40, 60, 80, and 120 μg/ml.Then the effect of 50 μg/ml rTCS alone or combined with 2 μΜ IL-2 on PC3 cell proliferation was analyzed. And the mechanism of rTCS was studied by western blot. After that the in vivo effect of rTCS combined with IL-2 was explored in mice bearing PC3 xenograft tumor. RESULTS: Trichosanthin was successfully expressed in BL21 and purified by 100 mM imidazole. It was shown to inhibit proliferation of PC3 cells in a dose-dependent manner with IC50 50.6 μg/ml. When combined with cytokine IL-2, a significant synergic effect was obtained. The inhibition rate on PC3 was around 50 % in combination group while only 35.5 % in single rTCS group at 50 μg/ml. Further, the expression of full length caspase-8 and Bcl-2 decreased significantly while cleaved caspase-8 and Bax were up-regulated, which suggest that caspase-8-mediated apoptosis pathway may be activated by rTCS in PC3 cells. Moreover, our data demonstrated that tumor volume and tumor weight were significantly reduced in rTCS-treated or rTCS/IL-2-treated nude mice bearing PC3 xenograft tumor compared with control. And significant difference was also found between rTCS and rTCS/IL-2 group. CONCLUSIONS: This study demonstrates that rTCS is a potential agent with high in vitro and in vivo anti-tumor activity on PC3 cells. And rTCS combined with IL-2 is a promising strategy in treating patients with prostate cancer in future.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100021en10.1186/s40659-016-0081-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Trichosanthin Interleukin IL-2 Prostate cancer cells PC3 Tumor
spellingShingle	Trichosanthin Interleukin IL-2 Prostate cancer cells PC3 Tumor Li,JinLong Li,Hui Zhang,ZhaoLi Wang,NianYue Zhang,Yong Chen The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
description	CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the role of trichosanthin on prostate cancer cells PC3. MATERIALS AND METHODS: Trichosanthin was expressed in BL21 strain and purified by affinity chromatography. MTT assay was designed to determine the effect of trichosanthin on growth of PC3 cells at doses of 10, 20, 40, 60, 80, and 120 μg/ml.Then the effect of 50 μg/ml rTCS alone or combined with 2 μΜ IL-2 on PC3 cell proliferation was analyzed. And the mechanism of rTCS was studied by western blot. After that the in vivo effect of rTCS combined with IL-2 was explored in mice bearing PC3 xenograft tumor. RESULTS: Trichosanthin was successfully expressed in BL21 and purified by 100 mM imidazole. It was shown to inhibit proliferation of PC3 cells in a dose-dependent manner with IC50 50.6 μg/ml. When combined with cytokine IL-2, a significant synergic effect was obtained. The inhibition rate on PC3 was around 50 % in combination group while only 35.5 % in single rTCS group at 50 μg/ml. Further, the expression of full length caspase-8 and Bcl-2 decreased significantly while cleaved caspase-8 and Bax were up-regulated, which suggest that caspase-8-mediated apoptosis pathway may be activated by rTCS in PC3 cells. Moreover, our data demonstrated that tumor volume and tumor weight were significantly reduced in rTCS-treated or rTCS/IL-2-treated nude mice bearing PC3 xenograft tumor compared with control. And significant difference was also found between rTCS and rTCS/IL-2 group. CONCLUSIONS: This study demonstrates that rTCS is a potential agent with high in vitro and in vivo anti-tumor activity on PC3 cells. And rTCS combined with IL-2 is a promising strategy in treating patients with prostate cancer in future.
author	Li,JinLong Li,Hui Zhang,ZhaoLi Wang,NianYue Zhang,Yong Chen
author_facet	Li,JinLong Li,Hui Zhang,ZhaoLi Wang,NianYue Zhang,Yong Chen
author_sort	Li,JinLong
title	The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
title_short	The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
title_full	The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
title_fullStr	The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
title_full_unstemmed	The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3
title_sort	anti-cancerous activity of recombinant trichosanthin on prostate cancer cell pc3
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100021
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The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3

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