Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques

BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities amo...

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Autores principales:	Traipe-Salas,Felipe, Traipe-Castro,Leonidas, Salinas-Toro,Daniela, López,Daniela, Valenzuela,Felipe, Cartes,Christian, Toledo-Araya,Héctor, Pérez,Claudio, Solís,Remigio López
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028
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spelling	oai:scielo:S0716-976020160001000282016-12-01Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniquesTraipe-Salas,FelipeTraipe-Castro,LeonidasSalinas-Toro,DanielaLópez,DanielaValenzuela,FelipeCartes,ChristianToledo-Araya,HéctorPérez,ClaudioSolís,Remigio López Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μ! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and darkfield. RESULTS: Fern-like structures (zones II and III) were visible with all illumination methods excepting brightfield. Zone I was the microdesiccate domain displaying the most noticeable illumination-dependent variations, namely transparent band delimited by an outer rim (Ph1, Ph2), homogeneous compactly built structure (brightfield) or invisible domain (darkfield, Ph3). Intermediate positions of the condenser (BF/Ph1, Ph1/Ph2) showed a structured roughly cylindrical zone I. The transition band also varied from invisibility (brightfield) to a well-defined domain comprising interwoven filamentous elements (phase contrasts, darkfield. CONCLUSIONS: Imaging of entire tear microdesiccates by transmitted-light microscopy depends upon illumination. A more comprehensive description of tear microdesiccates can be achieved by combining illumination methods.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028en10.1186/s40659-016-0089-0
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye
spellingShingle	Tear Tear ferning test Transmitted-light microscopy Tear microdesiccate Dry eye Traipe-Salas,Felipe Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Valenzuela,Felipe Cartes,Christian Toledo-Araya,Héctor Pérez,Claudio Solís,Remigio López Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
description	BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μ! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and darkfield. RESULTS: Fern-like structures (zones II and III) were visible with all illumination methods excepting brightfield. Zone I was the microdesiccate domain displaying the most noticeable illumination-dependent variations, namely transparent band delimited by an outer rim (Ph1, Ph2), homogeneous compactly built structure (brightfield) or invisible domain (darkfield, Ph3). Intermediate positions of the condenser (BF/Ph1, Ph1/Ph2) showed a structured roughly cylindrical zone I. The transition band also varied from invisibility (brightfield) to a well-defined domain comprising interwoven filamentous elements (phase contrasts, darkfield. CONCLUSIONS: Imaging of entire tear microdesiccates by transmitted-light microscopy depends upon illumination. A more comprehensive description of tear microdesiccates can be achieved by combining illumination methods.
author	Traipe-Salas,Felipe Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Valenzuela,Felipe Cartes,Christian Toledo-Araya,Héctor Pérez,Claudio Solís,Remigio López
author_facet	Traipe-Salas,Felipe Traipe-Castro,Leonidas Salinas-Toro,Daniela López,Daniela Valenzuela,Felipe Cartes,Christian Toledo-Araya,Héctor Pérez,Claudio Solís,Remigio López
author_sort	Traipe-Salas,Felipe
title	Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
title_short	Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
title_full	Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
title_fullStr	Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
title_full_unstemmed	Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
title_sort	progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028
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Progress in tear microdesiccate analysis by combining various transmitted-light microscope techniques

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