The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)

The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)

BACKGROUND: α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco...

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Autores principales: Liu,Yu, Wang,Lu, Liu,Heng, Zhao,Rongrong, Liu,Bin, Fu,Quanjuan, Zhang,Yuanhu
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2016
Materias:
Tobacco
Senescence
Transcriptome
Reactive oxygen species (ROS)
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100030
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spelling oai:scielo:S0716-976020160001000302016-12-01The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)Liu,YuWang,LuLiu,HengZhao,RongrongLiu,BinFu,QuanjuanZhang,Yuanhu Tobacco Senescence Transcriptome Reactive oxygen species (ROS) BACKGROUND: α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. RESULTS: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O2._) had higher levels in transgenics compared to controls. Transgenic plants also had enhanced sensitivity to oxidative stress. The transcriptome of 8-week-old plants was studied to detect molecular changes. Differentially expressed unigene analysis showed that ubiquitin-mediated proteolysis, cell growth, and death unigenes were upregulated. Unigenes related to photosynthesis, antioxidant activity, and nitrogen metabolism were downregulated. Combined with the expression analysis of senescence marker genes, these results indicate that senescence started in the leaves of the transgenic plants at the vegetative growth stage. CONCLUSIONS: The antioxidative defense system was compromised and the accumulation of reactive oxygen species (ROS) played an important role in the premature aging of transgenic plants.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100030en10.1186/s40659-016-0088-1
institution Scielo Chile
collection Scielo Chile
language English
topic Tobacco
Senescence
Transcriptome
Reactive oxygen species (ROS)
spellingShingle Tobacco
Senescence
Transcriptome
Reactive oxygen species (ROS)
Liu,Yu
Wang,Lu
Liu,Heng
Zhao,Rongrong
Liu,Bin
Fu,Quanjuan
Zhang,Yuanhu
The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
description BACKGROUND: α-Farnesene is a volatile sesquiterpene synthesized by the plant mevalonate (MVA) pathway through the action of α-farnesene synthase. The α-farnesene synthase 1 (MdAFS1) gene was isolated from apple peel (var. white winterpearmain), and transformed into tobacco (Nicotiana tabacum NC89). The transgenic plants had faster stem elongation during vegetative growth and earlier flowering than wild type (WT). Our studies focused on the transgenic tobacco phenotype. RESULTS: The levels of chlorophyll and soluble protein decreased and a lower seed biomass and reduced net photosynthetic rate (Pn) in transgenic plants. Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide radicals (O2._) had higher levels in transgenics compared to controls. Transgenic plants also had enhanced sensitivity to oxidative stress. The transcriptome of 8-week-old plants was studied to detect molecular changes. Differentially expressed unigene analysis showed that ubiquitin-mediated proteolysis, cell growth, and death unigenes were upregulated. Unigenes related to photosynthesis, antioxidant activity, and nitrogen metabolism were downregulated. Combined with the expression analysis of senescence marker genes, these results indicate that senescence started in the leaves of the transgenic plants at the vegetative growth stage. CONCLUSIONS: The antioxidative defense system was compromised and the accumulation of reactive oxygen species (ROS) played an important role in the premature aging of transgenic plants.
author Liu,Yu
Wang,Lu
Liu,Heng
Zhao,Rongrong
Liu,Bin
Fu,Quanjuan
Zhang,Yuanhu
author_facet Liu,Yu
Wang,Lu
Liu,Heng
Zhao,Rongrong
Liu,Bin
Fu,Quanjuan
Zhang,Yuanhu
author_sort Liu,Yu
title The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
title_short The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
title_full The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
title_fullStr The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
title_full_unstemmed The antioxidative defense system is involved in the premature senescence in transgenic tobacco (Nicotiana tabacum NC89)
title_sort antioxidative defense system is involved in the premature senescence in transgenic tobacco (nicotiana tabacum nc89)
publisher Sociedad de Biología de Chile
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100030
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