Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence

BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marthandan,Shiva, Menzel,Uwe, Priebe,Steffen, Groth,Marco, Guthke,Reinhard, Platzer,Matthias, Hemmerich,Peter, Kaether,Christoph, Diekmann,Stephan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Senescence Fibroblasts γ-irradiation Aging Transcriptome analysis DNA repair
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100034
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602016000100034
record_format	dspace
spelling	oai:scielo:S0716-976020160001000342016-12-01Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescenceMarthandan,ShivaMenzel,UwePriebe,SteffenGroth,MarcoGuthke,ReinhardPlatzer,MatthiasHemmerich,PeterKaether,ChristophDiekmann,Stephan Senescence Fibroblasts γ-irradiation Aging Transcriptome analysis DNA repair BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100034en10.1186/s40659-016-0095-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Senescence Fibroblasts γ-irradiation Aging Transcriptome analysis DNA repair
spellingShingle	Senescence Fibroblasts γ-irradiation Aging Transcriptome analysis DNA repair Marthandan,Shiva Menzel,Uwe Priebe,Steffen Groth,Marco Guthke,Reinhard Platzer,Matthias Hemmerich,Peter Kaether,Christoph Diekmann,Stephan Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
description	BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.
author	Marthandan,Shiva Menzel,Uwe Priebe,Steffen Groth,Marco Guthke,Reinhard Platzer,Matthias Hemmerich,Peter Kaether,Christoph Diekmann,Stephan
author_facet	Marthandan,Shiva Menzel,Uwe Priebe,Steffen Groth,Marco Guthke,Reinhard Platzer,Matthias Hemmerich,Peter Kaether,Christoph Diekmann,Stephan
author_sort	Marthandan,Shiva
title	Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
title_short	Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
title_full	Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
title_fullStr	Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
title_full_unstemmed	Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
title_sort	conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100034
work_keys_str_mv	AT marthandanshiva conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT menzeluwe conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT priebesteffen conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT grothmarco conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT guthkereinhard conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT platzermatthias conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT hemmerichpeter conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT kaetherchristoph conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence AT diekmannstephan conservedgenesandpathwaysinprimaryhumanfibroblaststrainsundergoingreplicativeandradiationinducedsenescence
_version_	1718441555199524864

Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence

Ejemplares similares