Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

BACKGROUND AND OBJECTIVE: Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. METHODS: Relativ...

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Autores principales: Han,Xu, Wang,Lingling, Ning,Yu, Li,Shuang, Wang,Zhenjun
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2016
Materias:
AFAP1-AS1
Colorectal cancer
Tumor metastasis
Epithelial-mesenchymal transition (EMT)
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100036
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spelling oai:scielo:S0716-976020160001000362016-12-01Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancerHan,XuWang,LinglingNing,YuLi,ShuangWang,Zhenjun AFAP1-AS1 Colorectal cancer Tumor metastasis Epithelial-mesenchymal transition (EMT) BACKGROUND AND OBJECTIVE: Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. METHODS: Relative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot. RESULTS: Relative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice. CONCLUSION: lncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100036en10.1186/s40659-016-0094-3
institution Scielo Chile
collection Scielo Chile
language English
topic AFAP1-AS1
Colorectal cancer
Tumor metastasis
Epithelial-mesenchymal transition (EMT)
spellingShingle AFAP1-AS1
Colorectal cancer
Tumor metastasis
Epithelial-mesenchymal transition (EMT)
Han,Xu
Wang,Lingling
Ning,Yu
Li,Shuang
Wang,Zhenjun
Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
description BACKGROUND AND OBJECTIVE: Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC. METHODS: Relative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot. RESULTS: Relative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice. CONCLUSION: lncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.
author Han,Xu
Wang,Lingling
Ning,Yu
Li,Shuang
Wang,Zhenjun
author_facet Han,Xu
Wang,Lingling
Ning,Yu
Li,Shuang
Wang,Zhenjun
author_sort Han,Xu
title Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
title_short Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
title_full Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
title_fullStr Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
title_full_unstemmed Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer
title_sort long non-coding rna afap1-as1 facilitates tumor growth and promotes metastasis in colorectal cancer
publisher Sociedad de Biología de Chile
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100036
work_keys_str_mv AT hanxu longnoncodingrnaafap1as1facilitatestumorgrowthandpromotesmetastasisincolorectalcancer
AT wanglingling longnoncodingrnaafap1as1facilitatestumorgrowthandpromotesmetastasisincolorectalcancer
AT ningyu longnoncodingrnaafap1as1facilitatestumorgrowthandpromotesmetastasisincolorectalcancer
AT lishuang longnoncodingrnaafap1as1facilitatestumorgrowthandpromotesmetastasisincolorectalcancer
AT wangzhenjun longnoncodingrnaafap1as1facilitatestumorgrowthandpromotesmetastasisincolorectalcancer
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