Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thym...

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Autores principales:	Lins,Marvin Paulo, Vieira,Larissa Fernanda de Araújo, Rosa,Alfredo Aurélio Marinho, Smaniotto,Salete
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Thymocyte Growth hormone Laminin Thymic epithelial cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100037
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spelling	oai:scielo:S0716-976020160001000372016-12-01Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitroLins,Marvin PauloVieira,Larissa Fernanda de AraújoRosa,Alfredo Aurélio MarinhoSmaniotto,Salete Thymocyte Growth hormone Laminin Thymic epithelial cells BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4-CD8-) and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+) thymocytes. CONCLUSIONS: The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100037en10.1186/s40659-016-0097-0
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Thymocyte Growth hormone Laminin Thymic epithelial cells
spellingShingle	Thymocyte Growth hormone Laminin Thymic epithelial cells Lins,Marvin Paulo Vieira,Larissa Fernanda de Araújo Rosa,Alfredo Aurélio Marinho Smaniotto,Salete Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
description	BACKGROUND: Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. RESULTS: GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4-CD8-) and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+) thymocytes. CONCLUSIONS: The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.
author	Lins,Marvin Paulo Vieira,Larissa Fernanda de Araújo Rosa,Alfredo Aurélio Marinho Smaniotto,Salete
author_facet	Lins,Marvin Paulo Vieira,Larissa Fernanda de Araújo Rosa,Alfredo Aurélio Marinho Smaniotto,Salete
author_sort	Lins,Marvin Paulo
title	Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
title_short	Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
title_full	Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
title_fullStr	Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
title_full_unstemmed	Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
title_sort	growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100037
work_keys_str_mv	AT linsmarvinpaulo growthhormoneinthepresenceoflamininmodulatesinteractionofhumanthymicepithelialcellsandthymocytesinvitro AT vieiralarissafernandadearaujo growthhormoneinthepresenceoflamininmodulatesinteractionofhumanthymicepithelialcellsandthymocytesinvitro AT rosaalfredoaureliomarinho growthhormoneinthepresenceoflamininmodulatesinteractionofhumanthymicepithelialcellsandthymocytesinvitro AT smaniottosalete growthhormoneinthepresenceoflamininmodulatesinteractionofhumanthymicepithelialcellsandthymocytesinvitro
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Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro

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