Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach

BACKGROUND: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healin...

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Autores principales:	Ilavenil,Soundharrajan, Kim,Da Hye, Vijayakumar,Mayakrishnan, Srigopalram,Srisesharam, Roh,Sang Gun, Arasu,Mariadhas Valan, Lee,Jong Suk, Choi,Ki Choon
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Chlorella vulgaris Chemical constituents Adipocyte differentiation PPAR-γ2 AMPK-a
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100038
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spelling	oai:scielo:S0716-976020160001000382016-12-01Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approachIlavenil,SoundharrajanKim,Da HyeVijayakumar,MayakrishnanSrigopalram,SrisesharamRoh,Sang GunArasu,Mariadhas ValanLee,Jong SukChoi,Ki Choon Chlorella vulgaris Chemical constituents Adipocyte differentiation PPAR-γ2 AMPK-a BACKGROUND: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healing substances. Type 2 diabetes mellitus is closely associated with adipogenesis and their factors. Hence, we aimed to investigate the chemical constituents and adipo-genic modulatory properties of C. vulgaris in 3T3-L1 pre-adipocytes. RESULTS: We analysed chemical constituents in ethanolic extract of C. vulgaris (EECV) by LC-MS. Results revealed that the EECV contains few triterpenoids and saponin compounds. Further, the effect of EECV on lipid accumulation along with genes and proteins expressions which are associated with adipogenesis and lipogenesis were evaluated using oil red O staining, qPCR and western blot techniques. The data indicated that that EECV treatment increased differentiation and lipid accumulation in 3T3-L1 cells, which indicates positive regulation of adipogenic and lipogenic activity. These increases were associated with up-regulation of PPAR-γ2, C/EBP-α, adiponectin, FAS, and leptin mRNA and protein expressions. Also, EECV treatments increased the concentration of glycerol releases as compared with control cells. Troglitazone is a PPAR-γ agonist that stimulates the PPAR-y2, adiponectin, and GLUT-4 expressions. Similarly, EECV treatments significantly upregulated PPAR-γ2, adiponectin, GLUT-4 expressions and glucose utilization. Further, EECV treatment decreased AMPK-α expression as compared with control and metformin treated cells. CONCLUSION: The present research findings confirmed that the EECV effectively modulates the lipid accumulation and differentiation in 3T3-L1 cells through AMPK-α mediated signalling pathway.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100038en10.1186/s40659-016-0098-z
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chlorella vulgaris Chemical constituents Adipocyte differentiation PPAR-γ2 AMPK-a
spellingShingle	Chlorella vulgaris Chemical constituents Adipocyte differentiation PPAR-γ2 AMPK-a Ilavenil,Soundharrajan Kim,Da Hye Vijayakumar,Mayakrishnan Srigopalram,Srisesharam Roh,Sang Gun Arasu,Mariadhas Valan Lee,Jong Suk Choi,Ki Choon Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
description	BACKGROUND: From ancient times, marine algae have emerged as alternative medicine and foods, contains the rich source of natural products like proteins, vitamins, and secondary metabolites, especially Chlorella vulgaris (C. vulgaris) contains numerous anti-inflammatory, antioxidants and wound healing substances. Type 2 diabetes mellitus is closely associated with adipogenesis and their factors. Hence, we aimed to investigate the chemical constituents and adipo-genic modulatory properties of C. vulgaris in 3T3-L1 pre-adipocytes. RESULTS: We analysed chemical constituents in ethanolic extract of C. vulgaris (EECV) by LC-MS. Results revealed that the EECV contains few triterpenoids and saponin compounds. Further, the effect of EECV on lipid accumulation along with genes and proteins expressions which are associated with adipogenesis and lipogenesis were evaluated using oil red O staining, qPCR and western blot techniques. The data indicated that that EECV treatment increased differentiation and lipid accumulation in 3T3-L1 cells, which indicates positive regulation of adipogenic and lipogenic activity. These increases were associated with up-regulation of PPAR-γ2, C/EBP-α, adiponectin, FAS, and leptin mRNA and protein expressions. Also, EECV treatments increased the concentration of glycerol releases as compared with control cells. Troglitazone is a PPAR-γ agonist that stimulates the PPAR-y2, adiponectin, and GLUT-4 expressions. Similarly, EECV treatments significantly upregulated PPAR-γ2, adiponectin, GLUT-4 expressions and glucose utilization. Further, EECV treatment decreased AMPK-α expression as compared with control and metformin treated cells. CONCLUSION: The present research findings confirmed that the EECV effectively modulates the lipid accumulation and differentiation in 3T3-L1 cells through AMPK-α mediated signalling pathway.
author	Ilavenil,Soundharrajan Kim,Da Hye Vijayakumar,Mayakrishnan Srigopalram,Srisesharam Roh,Sang Gun Arasu,Mariadhas Valan Lee,Jong Suk Choi,Ki Choon
author_facet	Ilavenil,Soundharrajan Kim,Da Hye Vijayakumar,Mayakrishnan Srigopalram,Srisesharam Roh,Sang Gun Arasu,Mariadhas Valan Lee,Jong Suk Choi,Ki Choon
author_sort	Ilavenil,Soundharrajan
title	Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
title_short	Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
title_full	Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
title_fullStr	Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
title_full_unstemmed	Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach
title_sort	potential role of marine algae extract on 3t3-l1 cell proliferation and differentiation: an in vitro approach
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100038
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Potential role of marine algae extract on 3T3-L1 cell proliferation and differentiation: an in vitro approach

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