Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer

BACKGROUND: Accumulating evidence has confirmed that miR-196a plays a critical role in tumorigenesis and tumor progression in a variety of cancers. It has been demonstrated that miR-196a is highly up-regulated in laryngeal cancer by miRNA profiling analysis. However, the functional mechanism of miR-...

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Autores principales:	Cheng,Jin, Yi,Zhang, Jiping,Li
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	miR-196a p27kp1 Laryngeal cancer
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100040
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spelling	oai:scielo:S0716-976020160001000402016-12-01Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancerCheng,JinYi,ZhangJiping,Li miR-196a p27kp1 Laryngeal cancer BACKGROUND: Accumulating evidence has confirmed that miR-196a plays a critical role in tumorigenesis and tumor progression in a variety of cancers. It has been demonstrated that miR-196a is highly up-regulated in laryngeal cancer by miRNA profiling analysis. However, the functional mechanism of miR-196a in laryngeal cancer remains unclear. This study aims to explore the mechanism of miR-196a in laryngeal cancer METHODS: In the present study, we conducted qPCR analysis of miR-196a expression in human laryngeal cancer and showed that miR-196a was overexpressed in tumor-derived samples and laryngeal cancer cell lines compared with matched normal controls. Further functional analysis of miR-196a demonstrated that the inhibition of miR-196a could inhibit laryngeal cell-cycle progression and proliferation in vitro. Luciferase reporter assay and western blot confirmed that miR-196a directly targeted p27kip1. Moreover, in order to investigate whether miR-196a regulated cell growth in laryngeal cancer cells by targeting p27kip1, rescue studies were performed in laryngeal cancer cells RESULTS: Results showed that overexpression of p27kip1 rescue decreased cell proliferation caused by miR-196a inhibitors. A negative relation between miR-196a and p27kip1 expression in laryngeal cancer tissues were also noted by further analyses CONCLUSIONS: The present study showed that miR-196a was upregulated in laryngeal cancer and promoted cell proliferation by downregulating p27kip1 in laryngeal cancer. However, further studies are needed to verify this findinginfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100040en10.1186/s40659-016-0100-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	miR-196a p27kp1 Laryngeal cancer
spellingShingle	miR-196a p27kp1 Laryngeal cancer Cheng,Jin Yi,Zhang Jiping,Li Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
description	BACKGROUND: Accumulating evidence has confirmed that miR-196a plays a critical role in tumorigenesis and tumor progression in a variety of cancers. It has been demonstrated that miR-196a is highly up-regulated in laryngeal cancer by miRNA profiling analysis. However, the functional mechanism of miR-196a in laryngeal cancer remains unclear. This study aims to explore the mechanism of miR-196a in laryngeal cancer METHODS: In the present study, we conducted qPCR analysis of miR-196a expression in human laryngeal cancer and showed that miR-196a was overexpressed in tumor-derived samples and laryngeal cancer cell lines compared with matched normal controls. Further functional analysis of miR-196a demonstrated that the inhibition of miR-196a could inhibit laryngeal cell-cycle progression and proliferation in vitro. Luciferase reporter assay and western blot confirmed that miR-196a directly targeted p27kip1. Moreover, in order to investigate whether miR-196a regulated cell growth in laryngeal cancer cells by targeting p27kip1, rescue studies were performed in laryngeal cancer cells RESULTS: Results showed that overexpression of p27kip1 rescue decreased cell proliferation caused by miR-196a inhibitors. A negative relation between miR-196a and p27kip1 expression in laryngeal cancer tissues were also noted by further analyses CONCLUSIONS: The present study showed that miR-196a was upregulated in laryngeal cancer and promoted cell proliferation by downregulating p27kip1 in laryngeal cancer. However, further studies are needed to verify this finding
author	Cheng,Jin Yi,Zhang Jiping,Li
author_facet	Cheng,Jin Yi,Zhang Jiping,Li
author_sort	Cheng,Jin
title	Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
title_short	Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
title_full	Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
title_fullStr	Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
title_full_unstemmed	Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
title_sort	upregulation of mir-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100040
work_keys_str_mv	AT chengjin upregulationofmir196apromotescellproliferationbydownregulatingp27kip1inlaryngealcancer AT yizhang upregulationofmir196apromotescellproliferationbydownregulatingp27kip1inlaryngealcancer AT jipingli upregulationofmir196apromotescellproliferationbydownregulatingp27kip1inlaryngealcancer
_version_	1718441556951695360

Upregulation of MiR-196a promotes cell proliferation by downregulating p27kip1 in laryngeal cancer

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