Metabotropic glutamate receptor 5 may be involved in macrophage plasticity

Abstract Background Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergi...

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Autores principales:	Shanshiashvili,Lali, Tsitsilashvili,Elene, Dabrundashvili,Nino, Kalandadze,Irine, Mikeladze,David
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Macrophages Metabotropic glutamate receptors Inflammation HMGB1 IL-10
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100203
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spelling	oai:scielo:S0716-976020170001002032017-04-10Metabotropic glutamate receptor 5 may be involved in macrophage plasticityShanshiashvili,LaliTsitsilashvili,EleneDabrundashvili,NinoKalandadze,IrineMikeladze,David Macrophages Metabotropic glutamate receptors Inflammation HMGB1 IL-10 Abstract Background Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells. Results Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2). Besides, our results have shown that RAW-mGluR5 macrophages expressed a higher level of peroxisome proliferator-activated receptor γ (PPAR-γ) and secreted more IL-10, high mobility group box 1 proteins (HMGB1) and Galectin-3 than control RAW-macrophages. Conclusions We propose that elevation of intracellular glutamate and expression of mGluR5 may initiate the metabolic rearrangement in macrophages that could contribute to the formation of an immunosuppressive phenotype.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100203en10.1186/s40659-017-0110-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Macrophages Metabotropic glutamate receptors Inflammation HMGB1 IL-10
spellingShingle	Macrophages Metabotropic glutamate receptors Inflammation HMGB1 IL-10 Shanshiashvili,Lali Tsitsilashvili,Elene Dabrundashvili,Nino Kalandadze,Irine Mikeladze,David Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
description	Abstract Background Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells. Results Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2). Besides, our results have shown that RAW-mGluR5 macrophages expressed a higher level of peroxisome proliferator-activated receptor γ (PPAR-γ) and secreted more IL-10, high mobility group box 1 proteins (HMGB1) and Galectin-3 than control RAW-macrophages. Conclusions We propose that elevation of intracellular glutamate and expression of mGluR5 may initiate the metabolic rearrangement in macrophages that could contribute to the formation of an immunosuppressive phenotype.
author	Shanshiashvili,Lali Tsitsilashvili,Elene Dabrundashvili,Nino Kalandadze,Irine Mikeladze,David
author_facet	Shanshiashvili,Lali Tsitsilashvili,Elene Dabrundashvili,Nino Kalandadze,Irine Mikeladze,David
author_sort	Shanshiashvili,Lali
title	Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
title_short	Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
title_full	Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
title_fullStr	Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
title_full_unstemmed	Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
title_sort	metabotropic glutamate receptor 5 may be involved in macrophage plasticity
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100203
work_keys_str_mv	AT shanshiashvililali metabotropicglutamatereceptor5maybeinvolvedinmacrophageplasticity AT tsitsilashvilielene metabotropicglutamatereceptor5maybeinvolvedinmacrophageplasticity AT dabrundashvilinino metabotropicglutamatereceptor5maybeinvolvedinmacrophageplasticity AT kalandadzeirine metabotropicglutamatereceptor5maybeinvolvedinmacrophageplasticity AT mikeladzedavid metabotropicglutamatereceptor5maybeinvolvedinmacrophageplasticity
_version_	1718441559026827264

Metabotropic glutamate receptor 5 may be involved in macrophage plasticity

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