Uptake of DNA by cancer cells without a transfection reagent

Abstract Background Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will hel...

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Autores principales:	Kong,Yanping, Zhang,Xianbo, Zhao,Yongliang, Xue,Yanfang, Zhang,Ye
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	DNA uptake Cancer cells Endocytosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100205
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spelling	oai:scielo:S0716-976020170001002052017-04-10Uptake of DNA by cancer cells without a transfection reagentKong,YanpingZhang,XianboZhao,YongliangXue,YanfangZhang,Ye DNA uptake Cancer cells Endocytosis Abstract Background Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis. Their uptake of DNAs might be different from normal cells; they may take in DNAs directly from the environment. In this report, we studied the uptake of DNAs in cancer cells without a transfection reagent. Methods A group of DNA fragments were prepared with PCR and labeled with isotope phosphorous-32 to test their uptake by Huh 7 (liver cancer) and THLE3 (normal liver cells) after incubation overnight by counting radioactivity of the cells’ genomic DNA. Multiple cell lines including breast cancer and lung cancer were tested with the same method. DNA molecules were also labeled with fluorescence to test the location in the cells using a kit of “label it fluorescence in situ hybridization (FISH)” from Mirus (USA). Results The data demonstrated that hepatocellular carcinoma cells possess the ability to take in large DNA fragments directly without a transfection reagent whereas normal liver cells cannot. Huh7 and MDA-MB231 cells displayed a significantly higher Rhodamine density in the cytoplasmic phagosomes and this suggests that the mechanism of uptake of large DNA by cancer cells is likely endocytosis. The efficacy of uptake is related to the DNA’s size. Some cell lines of lung cancer and breast cancer also showed similar uptake of DNA. Conclusions In the present study, we have revealed the evidence that some cancer cells, but not nontumorigenic cells, can take DNA fragments directly from the environment without the aid of the transfecting reagent.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100205en10.1186/s40659-017-0107-x
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA uptake Cancer cells Endocytosis
spellingShingle	DNA uptake Cancer cells Endocytosis Kong,Yanping Zhang,Xianbo Zhao,Yongliang Xue,Yanfang Zhang,Ye Uptake of DNA by cancer cells without a transfection reagent
description	Abstract Background Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Cancer cells are known for having gene mutations and the evolving ability of endocytosis. Their uptake of DNAs might be different from normal cells; they may take in DNAs directly from the environment. In this report, we studied the uptake of DNAs in cancer cells without a transfection reagent. Methods A group of DNA fragments were prepared with PCR and labeled with isotope phosphorous-32 to test their uptake by Huh 7 (liver cancer) and THLE3 (normal liver cells) after incubation overnight by counting radioactivity of the cells’ genomic DNA. Multiple cell lines including breast cancer and lung cancer were tested with the same method. DNA molecules were also labeled with fluorescence to test the location in the cells using a kit of “label it fluorescence in situ hybridization (FISH)” from Mirus (USA). Results The data demonstrated that hepatocellular carcinoma cells possess the ability to take in large DNA fragments directly without a transfection reagent whereas normal liver cells cannot. Huh7 and MDA-MB231 cells displayed a significantly higher Rhodamine density in the cytoplasmic phagosomes and this suggests that the mechanism of uptake of large DNA by cancer cells is likely endocytosis. The efficacy of uptake is related to the DNA’s size. Some cell lines of lung cancer and breast cancer also showed similar uptake of DNA. Conclusions In the present study, we have revealed the evidence that some cancer cells, but not nontumorigenic cells, can take DNA fragments directly from the environment without the aid of the transfecting reagent.
author	Kong,Yanping Zhang,Xianbo Zhao,Yongliang Xue,Yanfang Zhang,Ye
author_facet	Kong,Yanping Zhang,Xianbo Zhao,Yongliang Xue,Yanfang Zhang,Ye
author_sort	Kong,Yanping
title	Uptake of DNA by cancer cells without a transfection reagent
title_short	Uptake of DNA by cancer cells without a transfection reagent
title_full	Uptake of DNA by cancer cells without a transfection reagent
title_fullStr	Uptake of DNA by cancer cells without a transfection reagent
title_full_unstemmed	Uptake of DNA by cancer cells without a transfection reagent
title_sort	uptake of dna by cancer cells without a transfection reagent
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100205
work_keys_str_mv	AT kongyanping uptakeofdnabycancercellswithoutatransfectionreagent AT zhangxianbo uptakeofdnabycancercellswithoutatransfectionreagent AT zhaoyongliang uptakeofdnabycancercellswithoutatransfectionreagent AT xueyanfang uptakeofdnabycancercellswithoutatransfectionreagent AT zhangye uptakeofdnabycancercellswithoutatransfectionreagent
_version_	1718441559488200704

Uptake of DNA by cancer cells without a transfection reagent

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