Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells....

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Autores principales:	Mata-Miranda,Monica Maribel, Vazquez-Zapien,Gustavo Jesus, Rojas-Lopez,Marlon, Sanchez-Monroy,Virginia, Perez-Ishiwara,David Guillermo, Delgado-Macuil,Raul Jacobo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Pluripotent stem cells Differentiated kidney cells Vibrational spectroscopy Fourier transform infrared Principal components analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207
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spelling	oai:scielo:S0716-976020170001002072017-05-18Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cellsMata-Miranda,Monica MaribelVazquez-Zapien,Gustavo JesusRojas-Lopez,MarlonSanchez-Monroy,VirginiaPerez-Ishiwara,David GuillermoDelgado-Macuil,Raul Jacobo Pluripotent stem cells Differentiated kidney cells Vibrational spectroscopy Fourier transform infrared Principal components analysis Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. Results In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. Conclusion Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207en10.1186/s40659‑017‑0119‑6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pluripotent stem cells Differentiated kidney cells Vibrational spectroscopy Fourier transform infrared Principal components analysis
spellingShingle	Pluripotent stem cells Differentiated kidney cells Vibrational spectroscopy Fourier transform infrared Principal components analysis Mata-Miranda,Monica Maribel Vazquez-Zapien,Gustavo Jesus Rojas-Lopez,Marlon Sanchez-Monroy,Virginia Perez-Ishiwara,David Guillermo Delgado-Macuil,Raul Jacobo Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
description	Abstract Background Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. Results In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. Conclusion Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.
author	Mata-Miranda,Monica Maribel Vazquez-Zapien,Gustavo Jesus Rojas-Lopez,Marlon Sanchez-Monroy,Virginia Perez-Ishiwara,David Guillermo Delgado-Macuil,Raul Jacobo
author_facet	Mata-Miranda,Monica Maribel Vazquez-Zapien,Gustavo Jesus Rojas-Lopez,Marlon Sanchez-Monroy,Virginia Perez-Ishiwara,David Guillermo Delgado-Macuil,Raul Jacobo
author_sort	Mata-Miranda,Monica Maribel
title	Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
title_short	Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
title_full	Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
title_fullStr	Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
title_full_unstemmed	Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
title_sort	morphological, molecular and ftir spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100207
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Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

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