Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures

Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures

Abstract Objective To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures. Methods Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. Th...

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Autores principales: Ramachandran,Gokula Krishnan, Yeow,Chen Hua
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2017
Materias:
Nuclear magnetic resonance
Proton magnetic resonance spectrometry
Melanoma
Cancer
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100208
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spelling oai:scielo:S0716-976020170001002082017-05-18Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D culturesRamachandran,Gokula KrishnanYeow,Chen Hua Nuclear magnetic resonance Proton magnetic resonance spectrometry Melanoma Cancer Abstract Objective To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures. Methods Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed. Results In monolayer culture (2D), we observed one qualitative chemical shift marker for primary melanoma cells. In spheroid cultures (3D), we observed nine significant chemical shifts, of which eight markers were specific for primary melanoma spheroids, whereas the other one marker was specific to metastatic melanoma spheroids. This study suggests that the glucose accumulation and phospholipid composition vary significantly between the primary and metastatic cells lines that are obtained from a single donor and also with the cell culturing methods. 14 qualitative chemical shift markers were obtained in the comparison between monolayer culture and spheroids cultures irrespective of the differences in the cell lines. Among which 4 were unique to monolayer cultures whereas 10 chemical shifts were unique to the spheroid cultures. This study also shows that the method of cell culture would drastically affect the phospholipid composition of the cells and also depicts that the cells in spheroid culture closely resembles the cells in vivo. Conclusion This study shows the high specificity of proton NMR spectrometry in characterizing cancer cell lines and also shows the variations in the glucose accumulation and phospholipid composition between the primary and metastatic melanoma cell lines from the same donor. Differences in the cell culture method does plays an important role in phospholipid composition of the cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100208en10.1186/s40659-017-0117-8
institution Scielo Chile
collection Scielo Chile
language English
topic Nuclear magnetic resonance
Proton magnetic resonance spectrometry
Melanoma
Cancer
spellingShingle Nuclear magnetic resonance
Proton magnetic resonance spectrometry
Melanoma
Cancer
Ramachandran,Gokula Krishnan
Yeow,Chen Hua
Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
description Abstract Objective To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures. Methods Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed. Results In monolayer culture (2D), we observed one qualitative chemical shift marker for primary melanoma cells. In spheroid cultures (3D), we observed nine significant chemical shifts, of which eight markers were specific for primary melanoma spheroids, whereas the other one marker was specific to metastatic melanoma spheroids. This study suggests that the glucose accumulation and phospholipid composition vary significantly between the primary and metastatic cells lines that are obtained from a single donor and also with the cell culturing methods. 14 qualitative chemical shift markers were obtained in the comparison between monolayer culture and spheroids cultures irrespective of the differences in the cell lines. Among which 4 were unique to monolayer cultures whereas 10 chemical shifts were unique to the spheroid cultures. This study also shows that the method of cell culture would drastically affect the phospholipid composition of the cells and also depicts that the cells in spheroid culture closely resembles the cells in vivo. Conclusion This study shows the high specificity of proton NMR spectrometry in characterizing cancer cell lines and also shows the variations in the glucose accumulation and phospholipid composition between the primary and metastatic melanoma cell lines from the same donor. Differences in the cell culture method does plays an important role in phospholipid composition of the cells.
author Ramachandran,Gokula Krishnan
Yeow,Chen Hua
author_facet Ramachandran,Gokula Krishnan
Yeow,Chen Hua
author_sort Ramachandran,Gokula Krishnan
title Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
title_short Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
title_full Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
title_fullStr Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
title_full_unstemmed Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
title_sort proton nmr characterization of intact primary and metastatic melanoma cells in 2d & 3d cultures
publisher Sociedad de Biología de Chile
publishDate 2017
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100208
work_keys_str_mv AT ramachandrangokulakrishnan protonnmrcharacterizationofintactprimaryandmetastaticmelanomacellsin2damp3dcultures
AT yeowchenhua protonnmrcharacterizationofintactprimaryandmetastaticmelanomacellsin2damp3dcultures
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