Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats?
Abstract Background Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been report...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad de Biología de Chile
2017
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100209 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0716-97602017000100209 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0716-976020170001002092017-05-18Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats?Da,Yun‑MengNiu,Kai‑YuLiu,Shu‑YaWang,KeWang,Wen‑JuanJia,JingQin,Li‑HuaBai,Wen‑Pei Estradiol Cimicifuga racemosa Sublingual gland Subcellular structure Caspase-3 Abstract Background Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been reported whether C. racemosa has any impact on the sublingual gland, which may relate to dry mouth. In an attempt to determine this, we have compared the effects of estrogen and C. racemosa on the sublingual gland of ovariectomized rats. Results HE staining showed that the acinar cell area had contracted and that the intercellular spaces were broadened in the OVX (ovariectomized rats) group, while treatment with estradiol (E2) and iCR (isopropanolic extract of C. racemosa) improved these lesions. Transmission electron microscopy showed that rough endoplasmic reticulum expansion in mucous and serous acinar epithelial cells and apoptotic cells was more commonly seen in the OVX group than in the SHAM (sham-operated rats) group. Mitochondria and plasma membrane infolding lesions in the striated ducts were also observed. These lesions were alleviated by both treatments. It is of note that, in the OVX + iCR group, the volume of mitochondria in the striated duct was larger than in other groups. Immunohistochemical staining showed that the ratio of caspase-3 positive cells was significantly increased in the acinar cells of the OVX group compared with the SHAM group (p < 0.05); and the MA (mean absorbance) of caspase-3 in the striated ducts also increased (p < 0.05). Estradiol decreased the ratio of caspase-3 positive cells and the MA of caspase-3 in striated ducts significantly (p < 0.05). ICR also reduced the ratio of caspase-3 positive cells and the MA in the striated ducts (p < 0.05), but the reduction of the MA in striated ducts was inferior to that of the OVX + E2 group (p < 0.05). Conclusion Both estradiol and iCR can inhibit subcellular structural damage, and down-regulate the expression of caspase-3 caused by ovariectomy, but their effects were not identical, suggesting that both drugs confer a protective effect on the sublingual gland of ovariectomized rats, but that the specific location and mechanism of action producing these effects were different.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100209en10.1186/s40659-017-0115-x |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Estradiol Cimicifuga racemosa Sublingual gland Subcellular structure Caspase-3 |
| spellingShingle |
Estradiol Cimicifuga racemosa Sublingual gland Subcellular structure Caspase-3 Da,Yun‑Meng Niu,Kai‑Yu Liu,Shu‑Ya Wang,Ke Wang,Wen‑Juan Jia,Jing Qin,Li‑Hua Bai,Wen‑Pei Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| description |
Abstract Background Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been reported whether C. racemosa has any impact on the sublingual gland, which may relate to dry mouth. In an attempt to determine this, we have compared the effects of estrogen and C. racemosa on the sublingual gland of ovariectomized rats. Results HE staining showed that the acinar cell area had contracted and that the intercellular spaces were broadened in the OVX (ovariectomized rats) group, while treatment with estradiol (E2) and iCR (isopropanolic extract of C. racemosa) improved these lesions. Transmission electron microscopy showed that rough endoplasmic reticulum expansion in mucous and serous acinar epithelial cells and apoptotic cells was more commonly seen in the OVX group than in the SHAM (sham-operated rats) group. Mitochondria and plasma membrane infolding lesions in the striated ducts were also observed. These lesions were alleviated by both treatments. It is of note that, in the OVX + iCR group, the volume of mitochondria in the striated duct was larger than in other groups. Immunohistochemical staining showed that the ratio of caspase-3 positive cells was significantly increased in the acinar cells of the OVX group compared with the SHAM group (p < 0.05); and the MA (mean absorbance) of caspase-3 in the striated ducts also increased (p < 0.05). Estradiol decreased the ratio of caspase-3 positive cells and the MA of caspase-3 in striated ducts significantly (p < 0.05). ICR also reduced the ratio of caspase-3 positive cells and the MA in the striated ducts (p < 0.05), but the reduction of the MA in striated ducts was inferior to that of the OVX + E2 group (p < 0.05). Conclusion Both estradiol and iCR can inhibit subcellular structural damage, and down-regulate the expression of caspase-3 caused by ovariectomy, but their effects were not identical, suggesting that both drugs confer a protective effect on the sublingual gland of ovariectomized rats, but that the specific location and mechanism of action producing these effects were different. |
| author |
Da,Yun‑Meng Niu,Kai‑Yu Liu,Shu‑Ya Wang,Ke Wang,Wen‑Juan Jia,Jing Qin,Li‑Hua Bai,Wen‑Pei |
| author_facet |
Da,Yun‑Meng Niu,Kai‑Yu Liu,Shu‑Ya Wang,Ke Wang,Wen‑Juan Jia,Jing Qin,Li‑Hua Bai,Wen‑Pei |
| author_sort |
Da,Yun‑Meng |
| title |
Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| title_short |
Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| title_full |
Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| title_fullStr |
Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| title_full_unstemmed |
Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| title_sort |
does cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats? |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2017 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100209 |
| work_keys_str_mv |
AT dayun8209meng doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT niukai8209yu doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT liushu8209ya doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT wangke doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT wangwen8209juan doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT jiajing doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT qinli8209hua doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats AT baiwen8209pei doescimicifugaracemosahavetheeffectslikeestrogenonthesublingualglandinovariectomizedrats |
| _version_ |
1718441560521048064 |