Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro

Abstract Background Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. Methods T...

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Autores principales:	Gan,Zhihua, Han,Kun, Lin,Shuchen, Hu,Haiyan, Shen,Zan, Min,Daliu
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Cell cycle Migration Osteosarcoma Proliferation USP39
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210
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spelling	oai:scielo:S0716-976020170001002102017-05-18Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitroGan,ZhihuaHan,KunLin,ShuchenHu,HaiyanShen,ZanMin,Daliu Cell cycle Migration Osteosarcoma Proliferation USP39 Abstract Background Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. Methods The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test the impact of USP39-silencing on cellular proliferation, colony formation, cell cycle distribution and apoptosis. Results Knockdown of USP39 expression in U2OS cell significantly decreased cell proliferation, impaired colony formation ability. A further analysis indicated suppression of USP39 arrested cell cycle progression at G2/M phase via p21 dependent way. In addition, the results of Annexin V/7-AAD staining suggested the knockdown of USP39 could promote U2OS cell apoptosis through PARP cleavage. Conclusions These results uncover the critical role of USP39 in regulating cancer cell mitosis and indicate USP39 is critical for osteosarcoma tumorigenesis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210en10.1186/s40659-017-0121-z
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cell cycle Migration Osteosarcoma Proliferation USP39
spellingShingle	Cell cycle Migration Osteosarcoma Proliferation USP39 Gan,Zhihua Han,Kun Lin,Shuchen Hu,Haiyan Shen,Zan Min,Daliu Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
description	Abstract Background Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. Methods The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test the impact of USP39-silencing on cellular proliferation, colony formation, cell cycle distribution and apoptosis. Results Knockdown of USP39 expression in U2OS cell significantly decreased cell proliferation, impaired colony formation ability. A further analysis indicated suppression of USP39 arrested cell cycle progression at G2/M phase via p21 dependent way. In addition, the results of Annexin V/7-AAD staining suggested the knockdown of USP39 could promote U2OS cell apoptosis through PARP cleavage. Conclusions These results uncover the critical role of USP39 in regulating cancer cell mitosis and indicate USP39 is critical for osteosarcoma tumorigenesis.
author	Gan,Zhihua Han,Kun Lin,Shuchen Hu,Haiyan Shen,Zan Min,Daliu
author_facet	Gan,Zhihua Han,Kun Lin,Shuchen Hu,Haiyan Shen,Zan Min,Daliu
author_sort	Gan,Zhihua
title	Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
title_short	Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
title_full	Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
title_fullStr	Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
title_full_unstemmed	Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
title_sort	knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210
work_keys_str_mv	AT ganzhihua knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro AT hankun knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro AT linshuchen knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro AT huhaiyan knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro AT shenzan knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro AT mindaliu knockdownofubiquitinspecificpeptidase39inhibitedthegrowthofosteosarcomacellsandinducedapoptosisinvitro
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Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro

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