Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

Abstract Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, s...

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Autores principales:	Li,Pengfei, Yu,Xuejing, Xie,Jianshan, Yao,Xiaolei, Liu,Wenzhong, Yao,Jianbo, Zhu,Zhiwei, Lyu,Lihua
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Hen Follicles CART Theca cell Granulosa cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100211
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spelling	oai:scielo:S0716-976020170001002112017-06-14Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovaryLi,PengfeiYu,XuejingXie,JianshanYao,XiaoleiLiu,WenzhongYao,JianboZhu,ZhiweiLyu,Lihua Hen Follicles CART Theca cell Granulosa cell Abstract Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Methods: Small white follicles (1–2 mm in diameter) were treated for RNA isolation; Small white follicles (1–2 mm in diameter) and large white follicles (4–6 mm in diameter) were treated for immunohistochemical localization and large white follicles (4–6 mm in diameter), small yellow follicles (6–8 mm in diameter), large yellow follicles (9–12 mm in diameter), mature follicles (F5, F4, F3, F2, F1, >12 mm in diameter) were treated for RNA isolation and Real time PCR. Results: The results showed that full length of the CDS of hen CART was 336 bp encoding a 111 amino acid polypeptide. In the hen ovary, CART peptide was primarily localized to the theca layer, but not all, the oocyte and granulosa layer, with diffused, weaker staining than relative to the theca cell layer. Further, amount of CART mRNA was more (P < 0.05) in granulosa cells of 6–8 mm follicles compared with that in granulosa cells of other follicles. However, CART mRNA amount was greater in theca cells of 4–6 mm follicles relative to follicles of other sizes (P < 0.05). Conclusions: Results suggest that CART could play a potential role in developmental regulation of chicken follicles.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100211en10.1186/s40659-017-0123-x
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hen Follicles CART Theca cell Granulosa cell
spellingShingle	Hen Follicles CART Theca cell Granulosa cell Li,Pengfei Yu,Xuejing Xie,Jianshan Yao,Xiaolei Liu,Wenzhong Yao,Jianbo Zhu,Zhiwei Lyu,Lihua Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
description	Abstract Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Methods: Small white follicles (1–2 mm in diameter) were treated for RNA isolation; Small white follicles (1–2 mm in diameter) and large white follicles (4–6 mm in diameter) were treated for immunohistochemical localization and large white follicles (4–6 mm in diameter), small yellow follicles (6–8 mm in diameter), large yellow follicles (9–12 mm in diameter), mature follicles (F5, F4, F3, F2, F1, >12 mm in diameter) were treated for RNA isolation and Real time PCR. Results: The results showed that full length of the CDS of hen CART was 336 bp encoding a 111 amino acid polypeptide. In the hen ovary, CART peptide was primarily localized to the theca layer, but not all, the oocyte and granulosa layer, with diffused, weaker staining than relative to the theca cell layer. Further, amount of CART mRNA was more (P < 0.05) in granulosa cells of 6–8 mm follicles compared with that in granulosa cells of other follicles. However, CART mRNA amount was greater in theca cells of 4–6 mm follicles relative to follicles of other sizes (P < 0.05). Conclusions: Results suggest that CART could play a potential role in developmental regulation of chicken follicles.
author	Li,Pengfei Yu,Xuejing Xie,Jianshan Yao,Xiaolei Liu,Wenzhong Yao,Jianbo Zhu,Zhiwei Lyu,Lihua
author_facet	Li,Pengfei Yu,Xuejing Xie,Jianshan Yao,Xiaolei Liu,Wenzhong Yao,Jianbo Zhu,Zhiwei Lyu,Lihua
author_sort	Li,Pengfei
title	Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
title_short	Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
title_full	Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
title_fullStr	Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
title_full_unstemmed	Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary
title_sort	expression of cocaine- and amphetamine-regulated transcript (cart) in hen ovary
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100211
work_keys_str_mv	AT lipengfei expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT yuxuejing expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT xiejianshan expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT yaoxiaolei expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT liuwenzhong expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT yaojianbo expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT zhuzhiwei expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary AT lyulihua expressionofcocaineandamphetamineregulatedtranscriptcartinhenovary
_version_	1718441561005490176

Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

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