S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products

Abstract Background Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium‑binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xu,Yu‑Dong, Wang,Yu, Yin,Lei‑Miao, Peng,Ling‑Ling, Park,Gyoung‑Hee, Yang,Yong‑Qing
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end‑ products Platelet‑derived growth factor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100216
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602017000100216
record_format	dspace
spelling	oai:scielo:S0716-976020170001002162017-09-15S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-productsXu,Yu‑DongWang,YuYin,Lei‑MiaoPeng,Ling‑LingPark,Gyoung‑HeeYang,Yong‑Qing S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end‑ products Platelet‑derived growth factor Abstract Background Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium‑binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet‑derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Methods Rat ASMCs were cultured with or without a neutralizing antibody to the receptor for advanced glycation end‑products (RAGE), a potential receptor for S100A8 protein. Purified recombinant rat S100A8 protein was then added into the cultured cells, and the proliferation of ASMCs induced by PDGF was detected by colorimetric‑based WST‑8 assay and ampedance‑based xCELLigence proliferation assay. The expression levels of RAGE in ASMCs were analyzed using western blotting assay. Results Results showed that exogenous S100A8 inhibited the PDGF‑induced proliferation of rat ASMCs in a dose‑ dependent manner with the maximal effect at 1 μg/ml in vitro. Furthermore, when ASMCs was pre‑treated with anti‑RAGE neutralizing antibody, the inhibitory effect of S100A8 on PDGF‑induced proliferation was significantly sup‑ pressed. In addition, neither the treatment with S100A8 or PDGF alone nor the pre‑treatment with rS100A8 followed by PDGF stimulation affected the expression levels of RAGE. Conclusions Our study demonstrated that S100A8 inhibits PDGF‑induced ASMCs proliferation in a manner depend‑ ent on membrane receptor RAGE.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100216en10.1186/s40659-017-0128-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end‑ products Platelet‑derived growth factor
spellingShingle	S100A8 Airway smooth muscle cells Cell proliferation The receptor for advanced glycation end‑ products Platelet‑derived growth factor Xu,Yu‑Dong Wang,Yu Yin,Lei‑Miao Peng,Ling‑Ling Park,Gyoung‑Hee Yang,Yong‑Qing S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
description	Abstract Background Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium‑binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet‑derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Methods Rat ASMCs were cultured with or without a neutralizing antibody to the receptor for advanced glycation end‑products (RAGE), a potential receptor for S100A8 protein. Purified recombinant rat S100A8 protein was then added into the cultured cells, and the proliferation of ASMCs induced by PDGF was detected by colorimetric‑based WST‑8 assay and ampedance‑based xCELLigence proliferation assay. The expression levels of RAGE in ASMCs were analyzed using western blotting assay. Results Results showed that exogenous S100A8 inhibited the PDGF‑induced proliferation of rat ASMCs in a dose‑ dependent manner with the maximal effect at 1 μg/ml in vitro. Furthermore, when ASMCs was pre‑treated with anti‑RAGE neutralizing antibody, the inhibitory effect of S100A8 on PDGF‑induced proliferation was significantly sup‑ pressed. In addition, neither the treatment with S100A8 or PDGF alone nor the pre‑treatment with rS100A8 followed by PDGF stimulation affected the expression levels of RAGE. Conclusions Our study demonstrated that S100A8 inhibits PDGF‑induced ASMCs proliferation in a manner depend‑ ent on membrane receptor RAGE.
author	Xu,Yu‑Dong Wang,Yu Yin,Lei‑Miao Peng,Ling‑Ling Park,Gyoung‑Hee Yang,Yong‑Qing
author_facet	Xu,Yu‑Dong Wang,Yu Yin,Lei‑Miao Peng,Ling‑Ling Park,Gyoung‑Hee Yang,Yong‑Qing
author_sort	Xu,Yu‑Dong
title	S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
title_short	S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
title_full	S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
title_fullStr	S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
title_full_unstemmed	S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
title_sort	s100a8 inhibits pdgf-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100216
work_keys_str_mv	AT xuyu8209dong s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts AT wangyu s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts AT yinlei8209miao s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts AT pengling8209ling s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts AT parkgyoung8209hee s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts AT yangyong8209qing s100a8inhibitspdgfinducedproliferationofairwaysmoothmusclecellsdependentonthereceptorforadvancedglycationendproducts
_version_	1718441562156826624

S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products

Ejemplares similares