MiR-320 inhibits the growth of glioma cells through downregulating PBX3

Abstract Background MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in vari...

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Autores principales:	Pan,Cuicui, Gao,Hua, Zheng,Ni, Gao,Qi, Si,Yuanquan, Zhao,Yueran
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Glioma Luciferase reporter assay MiR-320 PBX3
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100224
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spelling	oai:scielo:S0716-976020170001002242017-11-03MiR-320 inhibits the growth of glioma cells through downregulating PBX3Pan,CuicuiGao,HuaZheng,NiGao,QiSi,YuanquanZhao,Yueran Glioma Luciferase reporter assay MiR-320 PBX3 Abstract Background MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Methods Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in the 3′ UTR of PBX3 in glioma cells U87 and U251. Increased miR-320 level in U87 and U251 cells was achieved through miR-320 mimic transfection and the effect of which on glioma cells growth, proliferation, cell cycle, apoptosis and activation of Raf-1/MAPK pathway was determined using MTT, colony formation, flow cytometry and western blot assays. PBX3 knockdown was performed using shPBX3 and the influence on MAPK pathway activation was evaluated. Results MiR-320 downregulation and PBX3 upregulation was found in glioma tissues. Luciferase reporter assays identified miR-320 directly blinds to the 3′ UTR of PBX3 in glioma cells. MiR-320 mimic transfection suppressed glioma cells proliferation, and induced cell cycle arrest and apoptosis. Both miR-320 overexpression and PBX3 knockdown inhibited Raf-1/MAPK activation. Conclusion MiR-320 may suppress glioma cells growth and induced apoptosis through the PBX3/Raf-1/MAPK axis, and miR-320 oligonucleotides may be a potential cancer therapeutic for glioma.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100224en10.1186/s40659-017-0137-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Glioma Luciferase reporter assay MiR-320 PBX3
spellingShingle	Glioma Luciferase reporter assay MiR-320 PBX3 Pan,Cuicui Gao,Hua Zheng,Ni Gao,Qi Si,Yuanquan Zhao,Yueran MiR-320 inhibits the growth of glioma cells through downregulating PBX3
description	Abstract Background MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Methods Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in the 3′ UTR of PBX3 in glioma cells U87 and U251. Increased miR-320 level in U87 and U251 cells was achieved through miR-320 mimic transfection and the effect of which on glioma cells growth, proliferation, cell cycle, apoptosis and activation of Raf-1/MAPK pathway was determined using MTT, colony formation, flow cytometry and western blot assays. PBX3 knockdown was performed using shPBX3 and the influence on MAPK pathway activation was evaluated. Results MiR-320 downregulation and PBX3 upregulation was found in glioma tissues. Luciferase reporter assays identified miR-320 directly blinds to the 3′ UTR of PBX3 in glioma cells. MiR-320 mimic transfection suppressed glioma cells proliferation, and induced cell cycle arrest and apoptosis. Both miR-320 overexpression and PBX3 knockdown inhibited Raf-1/MAPK activation. Conclusion MiR-320 may suppress glioma cells growth and induced apoptosis through the PBX3/Raf-1/MAPK axis, and miR-320 oligonucleotides may be a potential cancer therapeutic for glioma.
author	Pan,Cuicui Gao,Hua Zheng,Ni Gao,Qi Si,Yuanquan Zhao,Yueran
author_facet	Pan,Cuicui Gao,Hua Zheng,Ni Gao,Qi Si,Yuanquan Zhao,Yueran
author_sort	Pan,Cuicui
title	MiR-320 inhibits the growth of glioma cells through downregulating PBX3
title_short	MiR-320 inhibits the growth of glioma cells through downregulating PBX3
title_full	MiR-320 inhibits the growth of glioma cells through downregulating PBX3
title_fullStr	MiR-320 inhibits the growth of glioma cells through downregulating PBX3
title_full_unstemmed	MiR-320 inhibits the growth of glioma cells through downregulating PBX3
title_sort	mir-320 inhibits the growth of glioma cells through downregulating pbx3
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100224
work_keys_str_mv	AT pancuicui mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3 AT gaohua mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3 AT zhengni mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3 AT gaoqi mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3 AT siyuanquan mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3 AT zhaoyueran mir320inhibitsthegrowthofgliomacellsthroughdownregulatingpbx3
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MiR-320 inhibits the growth of glioma cells through downregulating PBX3

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