Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

Abstract Background Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this di...

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Autores principales:	González,Luis F., Henríquez-Belmar,Francisca, Delgado-Acevedo,Claudia, Cisternas-Olmedo,Marisol, Arriagada,Gloria, Sotomayor-Zárate,Ramón, Murphy,Dennis L., Moya,Pablo R.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive–compulsive disorder
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225
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spelling	oai:scielo:S0716-976020170001002252017-11-03Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous miceGonzález,Luis F.Henríquez-Belmar,FranciscaDelgado-Acevedo,ClaudiaCisternas-Olmedo,MarisolArriagada,GloriaSotomayor-Zárate,RamónMurphy,Dennis L.Moya,Pablo R. EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive–compulsive disorder Abstract Background Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain areas that are relevant to OCD. Results Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice. Conclusions Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225en10.1186/s40659-017-0138-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive–compulsive disorder
spellingShingle	EAAT3 SLC1A1 Neuronal glutamate transporter Obsessive–compulsive disorder González,Luis F. Henríquez-Belmar,Francisca Delgado-Acevedo,Claudia Cisternas-Olmedo,Marisol Arriagada,Gloria Sotomayor-Zárate,Ramón Murphy,Dennis L. Moya,Pablo R. Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
description	Abstract Background Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain areas that are relevant to OCD. Results Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice. Conclusions Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.
author	González,Luis F. Henríquez-Belmar,Francisca Delgado-Acevedo,Claudia Cisternas-Olmedo,Marisol Arriagada,Gloria Sotomayor-Zárate,Ramón Murphy,Dennis L. Moya,Pablo R.
author_facet	González,Luis F. Henríquez-Belmar,Francisca Delgado-Acevedo,Claudia Cisternas-Olmedo,Marisol Arriagada,Gloria Sotomayor-Zárate,Ramón Murphy,Dennis L. Moya,Pablo R.
author_sort	González,Luis F.
title	Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
title_short	Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
title_full	Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
title_fullStr	Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
title_full_unstemmed	Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
title_sort	neurochemical and behavioral characterization of neuronal glutamate transporter eaat3 heterozygous mice
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100225
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_version_	1718441564199452672

Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

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