Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells

Abstract Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has be...

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Autores principales:	Du,Maotao, Zhang,Zhong, Gao,Tao
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Piceatannol Apoptosis microRNA-181-a Melanoma cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226
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spelling	oai:scielo:S0716-976020170001002262018-01-08Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cellsDu,MaotaoZhang,ZhongGao,Tao Piceatannol Apoptosis microRNA-181-a Melanoma cells Abstract Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells. Methods Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific miRNA using Lipfectamine 2000. miRNA bingding ability to 3'-UTR region within specific gene was assed by firefly luciferase analysis. Gene and protein expression was eveluated by qRT-PCR and western blot analysis, respectively. Results Our study showed that piceatannol inhibited WM266-4 and A2058 cells growth and induced apoptosis. Totally, 16 differentially expressed miRNAs were screened out including 8 up-regulated and 8 down-regulated miRNAs. Expression level of miR-181a is significantly higher in piceatannol-treated cells than normal control and is lower in melanoma cancer tissues than its adjacent normal tissues. Bcl-2 is a target gene of miR-181a. Moreover, silencing of miR-181a reverses the decrease of cell viability induced by piceatannol in WM266-4 and A2058 cells. Taken together, present study uncovered the ability of piceatannol to repress melanoma cell growth and clarified the contribution of miR-181a in the anticancer role of piceatannol. Conclusion The present study proposes that piceatannol can be taken into account to be a hopeful anticancer agent for melanoma.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226en10.1186/s40659-017-0141-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Piceatannol Apoptosis microRNA-181-a Melanoma cells
spellingShingle	Piceatannol Apoptosis microRNA-181-a Melanoma cells Du,Maotao Zhang,Zhong Gao,Tao Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
description	Abstract Background Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells. Methods Melanoma cell lines WM266-4 and A2058 were treated either with or without piceatannol. Cell viability and cell apoptosis were assessed by using MTT and Annexin V/PI assay, respectively. Cells were transfected with specific miRNA using Lipfectamine 2000. miRNA bingding ability to 3'-UTR region within specific gene was assed by firefly luciferase analysis. Gene and protein expression was eveluated by qRT-PCR and western blot analysis, respectively. Results Our study showed that piceatannol inhibited WM266-4 and A2058 cells growth and induced apoptosis. Totally, 16 differentially expressed miRNAs were screened out including 8 up-regulated and 8 down-regulated miRNAs. Expression level of miR-181a is significantly higher in piceatannol-treated cells than normal control and is lower in melanoma cancer tissues than its adjacent normal tissues. Bcl-2 is a target gene of miR-181a. Moreover, silencing of miR-181a reverses the decrease of cell viability induced by piceatannol in WM266-4 and A2058 cells. Taken together, present study uncovered the ability of piceatannol to repress melanoma cell growth and clarified the contribution of miR-181a in the anticancer role of piceatannol. Conclusion The present study proposes that piceatannol can be taken into account to be a hopeful anticancer agent for melanoma.
author	Du,Maotao Zhang,Zhong Gao,Tao
author_facet	Du,Maotao Zhang,Zhong Gao,Tao
author_sort	Du,Maotao
title	Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
title_short	Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
title_full	Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
title_fullStr	Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
title_full_unstemmed	Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells
title_sort	piceatannol induced apoptosis through up-regulation of microrna-181a in melanoma cells
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100226
work_keys_str_mv	AT dumaotao piceatannolinducedapoptosisthroughupregulationofmicrorna181ainmelanomacells AT zhangzhong piceatannolinducedapoptosisthroughupregulationofmicrorna181ainmelanomacells AT gaotao piceatannolinducedapoptosisthroughupregulationofmicrorna181ainmelanomacells
_version_	1718441564376662016

Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells

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