Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile

Abstract Background: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner depen...

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Autores principales:	Patlán,Mariana, Sánchez-Muñoz,Fausto, Amezcua-Guerra,Luis M., Granados,Adriana, Páez,Araceli, Massó,Felipe, Mejía,Ana M., Soster,Angeles, Bojalil,Rafael, Pavón,Lenin, Jiménez-Zamudio,Luis A., Márquez-Velasco,Ricardo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Fresh frozen plasma Monocytes Cytokines Inflammation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100230
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spelling	oai:scielo:S0716-976020170001002302018-01-25Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profilePatlán,MarianaSánchez-Muñoz,FaustoAmezcua-Guerra,Luis M.Granados,AdrianaPáez,AraceliMassó,FelipeMejía,Ana M.Soster,AngelesBojalil,RafaelPavón,LeninJiménez-Zamudio,Luis A.Márquez-Velasco,Ricardo Fresh frozen plasma Monocytes Cytokines Inflammation Abstract Background: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors. Methods: FFP donors were stratified into groups of 19–30 years, 31–40 years or 41–50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide—LPS) for 24 h. Both in FFP and supernatants, TNF, IL-1β, IL-6, and IL-10 levels were measured by ELISA. Additionally, CD11B, TLR2, and CASP3 gene expression were measured by qtPCR in U937 cells. Total phagocytic activity was also assayed. Results: Elevated IL-10, but low TNF and IL-1β levels were measured in FFP from individuals aged 19–40 years, whereas in individuals aged 41–50 years FFP were characterized by equalized TNF and IL-10 levels. Elevated IL-6 levels were found in all FFP samples, especially in those from the oldest individuals. FFP stimulation was associated with striking modifications in cytokine production in an age-dependent way. Exposure to FFP attenuates the response to LPS. TLR2 and CD11B expression were enhanced regardless of the age of plasma donors, although CASP3 expression was increased only when FFP from individuals aged 19–40 years were tested. Phagocytosis decreased after exposure to FFP regardless of donor age. Conclusion: Our results suggest that soluble mediators in FFP may modulate the functioning of monocytes. Interestingly, this effect appears to be partially influenced by the age of donors.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100230en10.1186/s40659-017-0146-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fresh frozen plasma Monocytes Cytokines Inflammation
spellingShingle	Fresh frozen plasma Monocytes Cytokines Inflammation Patlán,Mariana Sánchez-Muñoz,Fausto Amezcua-Guerra,Luis M. Granados,Adriana Páez,Araceli Massó,Felipe Mejía,Ana M. Soster,Angeles Bojalil,Rafael Pavón,Lenin Jiménez-Zamudio,Luis A. Márquez-Velasco,Ricardo Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
description	Abstract Background: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors. Methods: FFP donors were stratified into groups of 19–30 years, 31–40 years or 41–50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide—LPS) for 24 h. Both in FFP and supernatants, TNF, IL-1β, IL-6, and IL-10 levels were measured by ELISA. Additionally, CD11B, TLR2, and CASP3 gene expression were measured by qtPCR in U937 cells. Total phagocytic activity was also assayed. Results: Elevated IL-10, but low TNF and IL-1β levels were measured in FFP from individuals aged 19–40 years, whereas in individuals aged 41–50 years FFP were characterized by equalized TNF and IL-10 levels. Elevated IL-6 levels were found in all FFP samples, especially in those from the oldest individuals. FFP stimulation was associated with striking modifications in cytokine production in an age-dependent way. Exposure to FFP attenuates the response to LPS. TLR2 and CD11B expression were enhanced regardless of the age of plasma donors, although CASP3 expression was increased only when FFP from individuals aged 19–40 years were tested. Phagocytosis decreased after exposure to FFP regardless of donor age. Conclusion: Our results suggest that soluble mediators in FFP may modulate the functioning of monocytes. Interestingly, this effect appears to be partially influenced by the age of donors.
author	Patlán,Mariana Sánchez-Muñoz,Fausto Amezcua-Guerra,Luis M. Granados,Adriana Páez,Araceli Massó,Felipe Mejía,Ana M. Soster,Angeles Bojalil,Rafael Pavón,Lenin Jiménez-Zamudio,Luis A. Márquez-Velasco,Ricardo
author_facet	Patlán,Mariana Sánchez-Muñoz,Fausto Amezcua-Guerra,Luis M. Granados,Adriana Páez,Araceli Massó,Felipe Mejía,Ana M. Soster,Angeles Bojalil,Rafael Pavón,Lenin Jiménez-Zamudio,Luis A. Márquez-Velasco,Ricardo
author_sort	Patlán,Mariana
title	Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
title_short	Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
title_full	Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
title_fullStr	Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
title_full_unstemmed	Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
title_sort	effect of fresh frozen plasma on the in vitro activation of u937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100230
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Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile

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