Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)

Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protoco...

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Autores principales:	Quiroz,Karla A., Berrios,Miguel, Carrasco,Basilio, Retamales,Jorge B., Caligari,Peter D. S., García-Gonzáles,Rolando
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404
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spelling	oai:scielo:S0716-976020170001004042017-07-07Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)Quiroz,Karla A.Berrios,MiguelCarrasco,BasilioRetamales,Jorge B.Caligari,Peter D. S.García-Gonzáles,Rolando Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3-6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions: For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404en10.1186/s40659-017-0125-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis
spellingShingle	Fragaria chiloensis Meristem culture Plant growth regulators Virus elimination Plant morphogenesis Quiroz,Karla A. Berrios,Miguel Carrasco,Basilio Retamales,Jorge B. Caligari,Peter D. S. García-Gonzáles,Rolando Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
description	Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a micropropagation protocol for F. chiloensis. The addition of polyvinylpyrrolidone (PVP) improved the meristem regeneration efficiency of F. chiloensis accessions. Similarly, the use of 6-benzylaminopurine (BAP) in the culture media increased the average rate of multiplication to 3-6 shoots per plant. In addition, the use of 6-benzylaminopurine (BAP), had low levels (near zero) of explant losses due to oxidation. However, plant height as well as number of leaves and roots were higher in media without growth regulators, with average values of 0.5 cm, 9 leaves and 4 roots per plant. Conclusions: For the first time in Chilean strawberry, meristem culture demonstrated to be an efficient tool for eliminating virus from infected plants, giving the possibility to produce disease free propagation material. Also, the addition of PVP into the basal MS medium improved the efficiency of plant recovery from isolated meristems. Farmers can now access to high quality plant material produced by biotech tools which will improve their technological practices.
author	Quiroz,Karla A. Berrios,Miguel Carrasco,Basilio Retamales,Jorge B. Caligari,Peter D. S. García-Gonzáles,Rolando
author_facet	Quiroz,Karla A. Berrios,Miguel Carrasco,Basilio Retamales,Jorge B. Caligari,Peter D. S. García-Gonzáles,Rolando
author_sort	Quiroz,Karla A.
title	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
title_short	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
title_full	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
title_fullStr	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
title_full_unstemmed	Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)
title_sort	meristem culture and subsequent micropropagation of chilean strawberry (fragaria chiloensis (l.) duch.)
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404
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Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)

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