In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis

Abstract Background Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the proce...

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Autores principales:	Diao,Ruiying, Cai,Xueyong, Liu,Lu, Yang,Lihua, Duan,YongGang, Cai,Zhiming, Gui,Yaoting, Mou,Lisha
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	CCR6 CCL20 Chemotaxis Sertoli cell Spermatogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100211
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spelling	oai:scielo:S0716-976020180001002112018-06-25In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesisDiao,RuiyingCai,XueyongLiu,LuYang,LihuaDuan,YongGangCai,ZhimingGui,YaotingMou,Lisha CCR6 CCL20 Chemotaxis Sertoli cell Spermatogenesis Abstract Background Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis. Methods and results CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis. Immunostaining results confirmed the localization of CCR 6 in testis. Further chemotaxis assays demonstrated that spermatogenic cells GC-1 and −2 exhibited a directional movement toward CCR6-specific ligand such as CCL20 or Sertoli cells in vitro. Conclusions The present findings indicate that CCR6 is involved in the chemotaxis of spermatogenic cells in vitro and promotes chemotaxis under non-inflammatory conditions during normal spermatogenesis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100211en10.1186/s40659-018-0161-z
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CCR6 CCL20 Chemotaxis Sertoli cell Spermatogenesis
spellingShingle	CCR6 CCL20 Chemotaxis Sertoli cell Spermatogenesis Diao,Ruiying Cai,Xueyong Liu,Lu Yang,Lihua Duan,YongGang Cai,Zhiming Gui,Yaoting Mou,Lisha In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
description	Abstract Background Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis. Methods and results CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis. Immunostaining results confirmed the localization of CCR 6 in testis. Further chemotaxis assays demonstrated that spermatogenic cells GC-1 and −2 exhibited a directional movement toward CCR6-specific ligand such as CCL20 or Sertoli cells in vitro. Conclusions The present findings indicate that CCR6 is involved in the chemotaxis of spermatogenic cells in vitro and promotes chemotaxis under non-inflammatory conditions during normal spermatogenesis.
author	Diao,Ruiying Cai,Xueyong Liu,Lu Yang,Lihua Duan,YongGang Cai,Zhiming Gui,Yaoting Mou,Lisha
author_facet	Diao,Ruiying Cai,Xueyong Liu,Lu Yang,Lihua Duan,YongGang Cai,Zhiming Gui,Yaoting Mou,Lisha
author_sort	Diao,Ruiying
title	In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
title_short	In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
title_full	In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
title_fullStr	In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
title_full_unstemmed	In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
title_sort	in vitro chemokine (c-c motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100211
work_keys_str_mv	AT diaoruiying invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT caixueyong invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT liulu invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT yanglihua invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT duanyonggang invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT caizhiming invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT guiyaoting invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis AT moulisha invitrochemokineccmotifreceptor6dependentnoninflammatorychemotaxisduringspermatogenesis
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In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis

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